C12E8 (Synonyms: Octaethylene Glycol Monododecyl Ether) |
Catalog No.GC43020
One-Click Copy Product Info
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C12E8 es un tensioactivo no iónico, su nombre completo es tensioactivo polioxietilenglicol, pertenece al tensioactivo de polioxieteleno éteno
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3055-98-9
Sample solution is provided at 25 µL, 10mM.
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C12E8 es un tensioactivo no iónico, su nombre completo es tensioactivo polioxietilenglicol, pertenece al tensioactivo de polioxieteleno éteno [1]. C12E8 regula la curvatura y la transición morfológica de la membrana vesicular de fosfolíoidos de una manera dependeinte de la concentración, promoviendo cambios morfológicos naturales a bajas concentraciones e induciendo una deformación anormal y, en última instancia, conduciendo a la desintegración de las vesículas de altas concentraciones[2-3].
En las células DC3F, C12E8 (0.05mg/mL; 45min) disminuye el voltaje requerido para la electroporación irreversible en bicapas lipídicas planas [4]. En los erythrocytes, se encontraron grandes invaginaciones /endovesicles planas con una región central comprimida y una periferia globular en secciones de eritrocitos tratados con C12E8 (44μM; 60 min) [5].
References:
[1]. Ruiz CC, Molina-Bolívar JA. Characterization of mixed non-ionic surfactants n-octyl-β-d-thioglucoside and octaethylene–glycol monododecyl ether: Micellization and microstructure. Journal of colloid and interface science. 2011 Sep 1; 361(1): 178-185.
[2]. Mavcic B, Babnik B, Iglic A, et al. Shape transformation of giant phospholipid vesicles at high concentrations of C12E8. Bioelectrochemistry. 2004 Jun 1; 63(1-2): 183-187.
[3]. Guo Y. Surface interactions between poly (ethylene glycol) and phospholipid bilayers: Effects on aggregation and fusion. State University of New York at Buffalo; 1993.
[4]. Kanduser M, Fosnaric M, Sentjurc M, et al. Effect of surfactant polyoxyethylene glycol (C12E8) on electroporation of cell line DC3F. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2003 Mar 12; 214(1-3): 205-217.
[5]. Bobrowska-Hägerstrand M, Kralj-Iglič V, Iglič A, et al. Torocyte membrane endovesicles induced by octaethyleneglycol dodecylether in human erythrocytes. Biophysical journal. 1999 Dec 1; 77(6): 3356-3362.
| Experimentos celulares [1]: | |
Líneas celulares | Células DC3F |
Método de preparación | Las células en suspensión se incubaron durante 45 minutos a 37℃; se aladió 0.05mg/mL de C12E8 a las células tratadas, mientras que el medio en el que se disolvió C12E8 se añadió a las células no tratadas. La fluidez de la membrana se midió mediante el método de resonancia paramagnética de electrones (EPR), utilizando la palmitato de 5-doxyl (MeFASL), que es lipofílico y , por lo tanto, se incorpora principalmente en la bicapa lipídica de membrana. Se colocó sesenta mililitros de 0.1mM de MeFASL en solución de etanol en el tubo de vidrio, luego se evaporó etanol por ratavapor. Se ñadió 1mL de suspensión celular que contenía 20 × 106 células a la película MeFASL formada en la pared del tubo de vidrio, y se incubó durante 15 minutos mientras se agitaba. Después de eso, la suspensión celular se centrifugó y el gránulo se colocó en un capilar de vidrio para las mediciones de EPR. A partir de los espectros EPR a 4℃, se midió la constante máxima de división hiperfina 2AII que refleja el parámetro de orden. |
Condiciones de reacción | 0.05mg/mL; 45 minutos |
Áreas de aplicación | C12E8 disminuye el voltaje requerido para la electroporación irreversible en bicapas lipídicas planas. |
References: | |
| Cas No. | 3055-98-9 | SDF | |
| Sinónimos | Octaethylene Glycol Monododecyl Ether | ||
| Canonical SMILES | CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO | ||
| Formula | C28H58O9 | M.Wt | 538.8 |
| Solubility | DMF: 30 mg/ml,DMSO: 15 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.856 mL | 9.2799 mL | 18.5598 mL |
| 5 mM | 371.2 μL | 1.856 mL | 3.712 mL |
| 10 mM | 185.6 μL | 928 μL | 1.856 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: An oil
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)