Cy5 NHS ester |
Catalog No.GC12907
One-Click Copy Product Info
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Cy5 NHS ester (Cyanine5 NHS ester) es un colorante fluorescente rojo para la marcación de grupos amino en péptidos, proteínas y oligonucleótidos.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Cy5 NHS ester (Cyanine5 NHS ester) es un colorante fluorescente rojo para la marcación de grupos amino en péptidos, proteínas y oligonucleótidos[1]. La excitación y emisión son a 646 y 669 nm, respectivamente[2].
Solución de Cy5 NHS ester a 10 mg/ml en DMSO. El colorante fluorescente rojo reactivo con aminas Cy5 NHS ester se utilizó para reaccionar con el grupo amino libre en el aminoácido cisteína presente en péptidos terminales usados para el recubrimiento final de esqueletos de pBAEs[1]. Por otro lado, 1 mg de colorante Cy5 NHS ester se disolvió en 0.1 ml de tampón tetraborato de sodio a 62.5 mM (pH 8.5). Después de añadir L-metionina en saturación, la mezcla se incubó durante la noche a 4°C. La metionina conjugada con fluoróforo (Cy5) (Cy5-Met) se purificó mediante cromatografía en fase inversa en un sistema HPLC de Agilent utilizando una columna C18[3].
References:
[1].Brugada-Vilà P, Cascante A, et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics. 2020 Feb 3;10(6):2744-2758.
[2].Jiménez Sánchez G, et al. Fluorescent Radiosensitizing Gold Nanoparticles. Int J Mol Sci. 2019 Sep 18;20(18):4618.
[3].Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642.
| Experimento de etiquetado [1]: | |
Método de Preparación | 1 mg del colorante Cy5 NHS éster fue disuelto en 0.1 ml de un buffer de tetraborato de sodio 62.5 mM (pH 8.5). Después de añadir L-metionina en saturación, la mezcla se incubó durante la noche a 4 °C. La metionina conjugada con el fluoróforo (Cy5-Met) se purificó mediante cromatografía en fase inversa en un sistema HPLC utilizando una columna C18. |
Condiciones de Reacción | 1 mg de Cy5 NHS en 0.1 ml de buffer de tetraborato de sodio |
Áreas de aplicación | La metionina conjugada con el fluoróforo se preparó mediante la conjugación del grupo amino alfa de la L-metionina con el colorante Cy5 NHS éster. |
Referencias: [1]. Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642. | |
| Cas No. | SDF | ||
| Chemical Name | (2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate | ||
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C | ||
| Formula | C36H40N3NaO10S2 | M.Wt | 761.84 |
| Solubility | very good in water, good in DMF and DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3126 mL | 6.5631 mL | 13.1261 mL |
| 5 mM | 262.5 μL | 1.3126 mL | 2.6252 mL |
| 10 mM | 131.3 μL | 656.3 μL | 1.3126 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
In vivo imaging of PDA NPs with or without PEI coating. Evolution of the fluorescent signals in the sham and CIA model mice after Cy5- labeled PDA and PEI-PDA NP treatment in the knee joints.
PDA NPs (20 mg/mL) were also covalently labeled with fluorescein isothiocyanate (0.2 mg/mL) or Cy5 succinimidyl ester (Cy5-NHS, GLPBIO, 0.2 mg/mL) to obtain FITC-PDA or Cy5-PDA NPs, respectively.
Bioactive Materials 24 (2023): 37-53. PMID: 36582350 IF: 16.8737 -
Related Biological Data
Retention of ACNs-UGCA-CMC in vivo. In vivo fluorescence images of mice and ex vivo fluorescence images of gastrointestinal tract and after oral administration of Cy5-NHS-labeled ACNs-UGCA-CMC (A) and mean fluorescence intensity in gastrointestinal tract.
Cy5-NHS-labeled(GLPBIO) ACNs-UGCA-CMC complexes were administered orally to the Kunming mice after a 12-hour fasting period at a dose of 1 mg/kg, while the control group received the same volume of physiological saline.
Food Res Int, 2024,176: 113804. PMID: 38163683 IF: 8.1
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)