ddGTP (2′,3′-Dideoxyguanosine 5′-triphosphate) trisodium is one of 2',3'-dideoxyribonucleoside 5'-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing. ddGTP trisodium acts as an inhibitor or a substrate for DNA polymerase α[1][2][3].
ddGTP trisodium inhibits the formation of both negative and positive DNA strands[1].ddGTP trisodium inhibits DNA polymerase α activity in the presence of Mn[2+] at low concentrations (<1 μM) [2]. ddGTP trisodium competitively inhibits the incorporation of [3H]dGTP into the DNA[2].
[1]. Debyser Z, et al. Kinetics of inhibition of endogenous human immunodeficiency virus type 1 reverse transcription by 2',3'-dideoxynucleoside 5'-triphosphate, tetrahydroimidazo-[4,5,1-jk][1,4]-benzodiazepin-2(1H)-thion e, and 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine derivatives. J Biol Chem. 1992 Jun 15;267(17):11769-76.
[2]. Ono K, et al. Utilization of 2',3'-dideoxyguanosine 5'-triphosphate as an inhibitor and substrate for DNA polymerase alpha. Biomed Pharmacother. 1990;44(2):115-21.
[3]. Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.