Astramembrangenin (Cycloastragenol) is a triterpenoid saponin compound and a hydrolysis product of the main active ingredient in Astragalus membranaceus (Fisch.) Bunge[1].Astramembrangenin is orally safe and has broad Extensive pharmacological effects, including telomerase activation, telomere elongation, anti-inflammatory and anti-oxidative properties[2].Astramembrangenin has antiaging properties, CAG stimulates telomerase activity in human neonatal keratinocytes and rat neuronal cells, and induces CREB activation followed by tert and bcl2 expression. Cycloastragenol (CAG) may have a novel therapeutic role in depression[2].
Astramembrangenin (0-10 µM; 3-6 days) increases cell growth compared to the vehicle control in HEK cultures[1].Astramembrangenin (0.3 µM; 5-90 mins) induces CREB phosphorylation in primary cortical neurons, total CREB expression is unaltered by CAG in both cell type[1].Astramembrangenin (3 µM; 6-48 hours) may promotes prosurvival signaling in neurons, increases tert mRNA expression, exhibits increased bcl2 mRNA expression[1]. Cell Viability Assay[1] Cell Line: HEK cells
[1]. Ip FC, et al. Cycloastragenol is a potent telomerase activator in neuronal cells: implications for depression management. Neurosignals. 2014;22(1):52-63. [2]. Yu Y, et al. Cycloastragenol: An exciting novel candidate for age-associated diseases. Exp Ther Med. 2018 Sep;16(3):2175-2182. [3]. Sun C, et al. Cycloastragenol mediates activation and proliferation suppression in concanavalin A-induced mouse lymphocyte pan-activation model. Immunopharmacol Immunotoxicol. 2017 Jun;39(3):131-139.