Maurocalcine TFA is an agonist of ryanodine receptor (RyR) channel types 1, 2 and 3 with cellular permeability. Maurocalcine TFA induces [3H]ryanodine binding on RyR1 with an EC50 value of 2558 nM. Maurocalcine TFA exhibits a apparent affinity of 14 nM for RyR2. Maurocalcine TFA can be applied to in vivo cell tracking or other cell imaging techniques.
Maurocalcine (100 nM, 24 h) increases the sensitivity of RyR2 to activating [Ca2+]i and decrease its sensitivity to inhibiting [Ca2+]i[1].Maurocalcine (5 μM, 4 h/24 h) is absolutely no sign of significant cell toxicity for HEK293[3].
References:
[1]. De Waard S, et al. Maurocalcin and its analog MCaE12A facilitate Ca2+ mobilization in cardiomyocytes. Biochem J. 2020 Oct 30;477(20):3985-3999.
[2]. Boisseau S, et al. Cell penetration properties of maurocalcine, a natural venom peptide active on the intracellular ryanodine receptor. Biochim Biops Acta. 2006 Mar;1758(3):308-19. [3]. Boisseau S, et al. Cell penetration properties of maurocalcine, a natural venom peptide active on the intracellular ryanodine receptor. Biochim Biops Acta. 2006 Mar;1758(3):308-19.