Reactive Probes

MTSSL is a paramagnetic nitroxide spin label featuring a methanethiosulfonate group for site-directed spin labeling, particularly through disulfide linkages with cysteine residues on proteins.

Direct Red 80 (Sirius Red) es un colorante poliazoico utilizado principalmente en métodos de tinciÓn para colÁgeno y amiloide.

YnMyr Diazirine X10 is a cell-permeable photoreactive probe for protein myristoylation.

12(S)-HETE is the predominant lipoxygenase product of mammalian platelets.

4-oxo-2-Nonenal is a product of lipid peroxidation that actively modifies histidine and lysine residues on proteins and causes protein cross-linking.

α-D-Galactopyranosylphenyl isothiocyanate is a chemically activated form of galactose that has been used to prepare various neoglycoproteins, which consist of a glycosylated serum albumin substituted with either fluorescein or methotrexate.

S-Nitrosylation reactions regulate protein function and mediate nitrosative stress.

Redox-sensitive cysteine residues in proteins may function as sensors of reactive oxygen species (ROS) and also serve as molecular switches, activating or deactivating proteins, following a change in oxidation state.

BCN-E-BCN is a strained cycloalkyne probe for detecting proteins that have been sulfenylated, the first intermediate step in protein oxidation.

A hydrophobic spin label

4'-Aminomethyl-4,5',8-trimethylpsoralen is a bifunctional photoreactive agent for crosslinking nucleic acids.

9,10-Anthracenediyl-bis(methylene)dimalonic Acid es un derivado soluble en agua del antraceno y se aplica generalmente como sonda de oxígeno singlet. 9,10-Anthracenediyl-bis(methylene)dimalonic Acid puede ser fotoblanqueado por oxígeno singlete a su endoperóxido correspondiente, causando una disminución dependiente del tiempo en la absorbancia a 380-400nm

El ADN es continuamente dañado por agentes endógenos y ambientales, lo que lleva a la formación de sitios abásicos (apurínicos/apirimidínicos, AP) que son disruptivos para la síntesis del ADN.

DAz-2 is a cell-permeable chemical probe used to detect cysteine oxidation in proteins.

cDEPMPO es un potente profÁrmaco contra el cÁncer. Las altas concentraciones de DEPMPO atrapan por rotaciÓn el radical .OH junto con los radicales arilo que surgen de compuestos BTO (benzotriazina 1,4-di-N-Óxido) reducidos con un electrÓn.

Disuccinimidyl Sulfoxide (DSSO) es un reticulante homofuncional, dirigido a la amina, que contiene sulfoxide utilizando para el análisis de las interacciones proteína-proteína (PPIs) mediante espectromatría de masas de reticulación (XL-MS).

IBTP is a lipophilic cation that is accumulated in mitochondria and forms stable thioether adducts in a thiol-specific manner.

MTSEA-biotin is a thiol-reactive probe that can be used to label proteins at cysteine residues.

Methanethiosulfonates (MTS) are sulfhydryl-reactive compounds that form mixed disulfide linkages and are commonly used to study cysteine residues on proteins.

MTSET (chloride) is a methanethiosulfonate (MTS), a sulfhydryl-reactive compound that forms mixed disulfide linkages.

Labeling reactive centers of various types in cells with specific site-directed probes is a common method to explore both function and biochemical modification of proteins.

SNOB 1 Reagent is a biotinylated probe for detecting S-nitrosylated proteins in a single step.

An internal standard for the quantification of Girard’s reagent P

La 5-etiniluridina (5-EU) es un potente nucleÓsido permeable a las células que se puede utilizar para marcar el ARN recién sintetizado.