DEPMPO (Synonyms: 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide) |
| Catalog No.GC43410 |
cDEPMPO es un potente profÁrmaco contra el cÁncer. Las altas concentraciones de DEPMPO atrapan por rotaciÓn el radical .OH junto con los radicales arilo que surgen de compuestos BTO (benzotriazina 1,4-di-N-Óxido) reducidos con un electrÓn.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 157230-67-6
Sample solution is provided at 25 µL, 10mM.
DEPMPO is a phosphorylated derivative of the widely used DMPO spin trap. It has been reported to produce spin adducts with increased stability particularly for the adduct of superoxide. [1] [2] [3] DEPMPO displays a detectable spin adduct signal at a concentration as low as 1 mM, as compared to 5 mM for DMPO.[3] A significant advantage of using DEPMPO in trapping superoxide radical is that the decomposition of DEPMPO/O2•- does not produce the OH• adduct, which can be a drawback when using DMPO. [2][3]
Reference:
[1]. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).
[2]. Anzai, K., Aikawa, T., Furukawa, Y., et al. ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes. Archives of Biochemistry and Biophysics 415, 251-256 (2003).
[3]. Liu, K.J., Miyake, M., Panz, T., et al. Evaluation of DEPMPO as a spin trapping agent in biological systems. Free Radical Biology & Medicine 26, 714-721 (1999).
| Cas No. | 157230-67-6 | SDF | |
| Sinónimos | 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide | ||
| Chemical Name | P-(3,4-dihydro-2-methyl-1-oxido-2H-pyrrol-2-yl)-phosphonic acid, diethyl ester | ||
| Canonical SMILES | [O-][N+]1=CCCC1(P(OCC)(OCC)=O)C | ||
| Formula | C9H18NO4P | M.Wt | 235.2 |
| Solubility | Soluble in water to 100 mM, in ethanol and in DMSO to 25 mM | Storage | Store at -80°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.2517 mL | 21.2585 mL | 42.517 mL |
| 5 mM | 0.8503 mL | 4.2517 mL | 8.5034 mL |
| 10 mM | 0.4252 mL | 2.1259 mL | 4.2517 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
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