Streptomycin |
Catalog No.GC19579
One-Click Copy Product Info
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Streptomycin is an effective antibiotic against Mycobacterium tuberculosis and is used in the study of tuberculosis (TB) .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 57-92-1
Sample solution is provided at 25 µL, 10mM.
Streptomycin is an effective antibiotic against Mycobacterium tuberculosis and is used in the study of tuberculosis (TB)[1]. Streptomycin acts as a protein synthesis inhibitor, binding irreversibly to the small 16S rRNA of the 30S ribosomal subunit, interfering with protein synthesis [2]. Streptomycin originally had broad Gram-negative and Gram-positive coverage, but its spectrum of activity has been significantly narrowed due to antibiotic resistance [3]. Streptomycin is an aminoglycoside antibacterial drug with poor oral absorption and can be administered intramuscularly or intravenously [4].
In vitro, treatment of guinea pig ventricular myocytes with streptomycin (40 μM) for 2 min significantly inhibited the stretch-induced increase in Ca2+. The decrease in Ca2+ occurred 18 seconds after the application of streptomycin (n=13 cells). In all cells, the decrease in Ca2+ occurred in less than 60 seconds, indicating that streptomycin has a calcium homeostatic effect on cardiomyocytes [5]. Streptomycin (5, 50μg/ml) can effectively inhibit the survival and proliferation of Mycobacterium tuberculosis in human macrophages infected with Mycobacterium tuberculosis[6].
In vivo, Streptomycin (50-100 mg/kg) was used to treat mice infected with Mycobacterium avium complex (MAC) by intramuscular injection for 4 weeks, which significantly reduced the MAC bacterial load in the spleen and liver, and showed partial protection in histopathological examination, but did not completely eliminate the infection. The liposome encapsulation effect of Streptomycin was better than that of the free form[7].
References:
[1] Cohen K A, Stott K E, Munsamy V, et al. Evidence for expanding the role of streptomycin in the management of drug-resistant Mycobacterium tuberculosis[J]. Antimicrobial agents and chemotherapy, 2020, 64(9): 10.1128/aac. 00860-20.
[2] Weisblum B, Davies J. Antibiotic inhibitors of the bacterial ribosome[J]. Bacteriological reviews, 1968, 32(4_pt_2): 493-528.
[3] Guardabassi L, Courvalin P. Modes of antimicrobial action and mechanisms of bacterial resistance[J]. Antimicrobial resistance in bacteria of animal origin, 2005: 1-18.
[4] EDSON R S, TERRELL C L. The aminoglycosides: streptomycin, kanamycin, gentamicin, tobramycin, amikacin, netilmicin, and sisomicin[C]//Mayo Clinic Proceedings. Elsevier, 1987, 62(10): 916-920.
[5] Gannier F, White E, Lacampagne A, et al. Streptomycin reverses a large stretch induced increase in [Ca2+] i in isolated guinea pig ventricular myocytes[J]. Cardiovascular research, 1994, 28(8): 1193-1198.
[6] Crowle A J, Sbarbaro J A, Judson F N, et al. Inhibition by streptomycin of tubercle bacilli within cultured human macrophages[J]. American Review of Respiratory Disease, 1984, 130(5): 839-844.
[7] Gangadharam P R J, Ashtekar D A, Ghori N, et al. Chemotherapeutic potential of free and liposome encapsulated streptomycin against experimental Mycobacterium avium complex infections in beige mice[J]. Journal of Antimicrobial Chemotherapy, 1991, 28(3): 425-435.
| Cell experiment [1]: | |
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Cell lines |
Guinea pig ventricular myocytes |
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Preparation Method |
Ventricular myocytes were treated with 40µM Streptomycin for 2 minutes to apply mechanical stretch to the cells to simulate the mechanical stress during cardiac contraction and relaxation. Changes in intracellular Ca2+ concentration were recorded at different time points. |
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Reaction Conditions |
40μM; 2min |
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Applications |
Application of streptomycin to this sensitive cell rapidly reversed the increase in Ca2+. The decrease in Ca2+ occurred 18 seconds after application of streptomycin (n = 13 cells). In all cells, the decrease in Ca2+ occurred in less than 60 seconds. |
| Animal experiment [2]: | |
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Animal models |
Beige mice |
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Preparation Method |
Beige mice were injected with Mycobacterium avium complex (MAC) suspension via the tail vein to establish a systemic infection model. Two doses(50-100 mg/kg) of free streptomycin, were given daily intramuscularly (im), five days a week for four weeks. In comparison, the drug encapsulated in large multilaminar liposomes was investigated at 5, 10 and15 mg/kb dose and administered iv only in two doses at day 1 and day 7 following the infection. Control groups received buffer, buffer-loaded d vesicles or free streptomycin given iv (15 mg/kg) every week for the four week durat tion. |
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Dosage form |
100mg/kg; i.m. |
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Applications |
Streptomycin exhibits significant antibacterial effects against MAC infection in beige mice, with liposome-encapsulated Streptomycin being more effective in reducing bacterial load and tissue lesions. |
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References: [1] Gannier F, White E, Lacampagne A, et al. Streptomycin reverses a large stretch induced increase in [Ca2+] i in isolated guinea pig ventricular myocytes[J]. Cardiovascular research, 1994, 28(8): 1193-1198. [2]Gangadharam P R J, Ashtekar D A, Ghori N, et al. Chemotherapeutic potential of free and liposome encapsulated streptomycin against experimental Mycobacterium avium complex infections in beige mice[J]. Journal of Antimicrobial Chemotherapy, 1991, 28(3): 425-435. |
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| Cas No. | 57-92-1 | SDF | |
| Formula | C21H39N7O12 | M.Wt | 581.57 |
| Solubility | H2O : >100 mg/mL (171.94 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7195 mL | 8.5974 mL | 17.1948 mL |
| 5 mM | 343.9 μL | 1.7195 mL | 3.439 mL |
| 10 mM | 171.9 μL | 859.7 μL | 1.7195 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)