Trypaflavin is an acridine compound and antimalarial agent. Trypaflavin invades germ cells. Trypaflavin induces aberrations in unfertilized oocytes. Trypaflavin increases the frequency of chromosomal aberrations. Trypaflavin shows weak mutagenicity. Trypaflavin is highly toxic to Leishmania, causing immediate lysis of the leptomonads [1] [2].
In Vivo, Trypaflavin (50 mg/kg; p.o.; single acute dose; day 7 post conception) induces dominant lethal mutations in F1 female offspring of pregnant C3H mice, with an induced mutation frequency of 13.25[1]. Trypaflavin (2 mg/kg/day; p.o.; 50 days) slightly elevates preimplantation egg loss and dead implants without inducing significant dominant lethal mutations in female C3H mice, and weakly increases total chromosome aberration frequencies to 21.1% in metaphase-II oocytes of female NMRI mice[1].
References:
[1]. Basler A, et al. Comparative investigations with trypaflavin in metaphase-II oocytes and in dominant lethal assay. Hum Genet. 1977 Dec 29;40(1):87-92.
[2]. Senekji H A. The effect of sulfanilamide and trypaflavin on cultures of Leishmania tropica. Journal of Infectious Diseases, 1940, 66(2): 111-112.
In Vivo, Trypaflavin (50 mg/kg; p.o.; single acute dose; day 7 post conception) induces dominant lethal mutations in F1 female offspring of pregnant C3H mice, with an induced mutation frequency of 13.25[1]. Trypaflavin (2 mg/kg/day; p.o.; 50 days) slightly elevates preimplantation egg loss and dead implants without inducing significant dominant lethal mutations in female C3H mice, and weakly increases total chromosome aberration frequencies to 21.1% in metaphase-II oocytes of female NMRI mice[1].
References:
[1]. Basler A, et al. Comparative investigations with trypaflavin in metaphase-II oocytes and in dominant lethal assay. Hum Genet. 1977 Dec 29;40(1):87-92.
[2]. Senekji H A. The effect of sulfanilamide and trypaflavin on cultures of Leishmania tropica. Journal of Infectious Diseases, 1940, 66(2): 111-112.