2×Taq PCR Master Mix (with blue dye) is a ready-to-use, rapid and simple PCR pre-mixed solution that contains blue loading buffer. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up.
The reaction mixture only needs to add primers, template, and ddH2O. 2×Taq PCR Master Mix contains blue loading buffer already, which saved the step of mix loading buffer, and could indicate the electrophoresis progress after the PCR reaction. The migration speed of the blue dye in 1% TAE agarose gel is similar to a 500 bp double-stranded DNA fragment.
Taq DNA polymerase has no 3'→5' exonuclease activity and generates PCR products with 3'-dA overhangs, which can be directly used for TA vector cloning after purification. It is recommended to use 2×Taq PCR Fusion-Fast Mix (with blue dye) (catalog number: GK10036) when the GC content of the target fragment is high (>60%) or the target fragment is too much long.
2×Taq PCR Master Mix (with blue dye) can be used for large-scale gene detection, semi-quantitative PCR experiments, and the detection of trace amounts of DNA after PCR amplification, as well as for amplifying PCR products from complex templates such as genomes, for example, for cloning expressed genes, gene point mutations (SNP) analysis, and gene targeted mutations.
Note: Template dosage: 10-1000 ng genomic DNA; 1 to 30 ng of plasmid or 1 to 2 μl of cDNA after RT-PCR reaction. The above example is a conventional PCR reaction system, which is for reference only. The actual reaction conditions vary with different structures such as templates and primers. The best reaction conditions should be set according to the characteristics of templates, primers and target fragments, and the reaction system should be enlarged or reduced according to the proportion.