2×Taq PCR Super Fast Mix(with blue dye) is a ready-to-use, rapid, and simple pre-mixed solution for PCR that contains a blue loading buffer, and it is suited for super-fast PCR reactions.
The reaction mixture only needs to add primers, template, and ddH2O. 2×Taq PCR Master Mix contains blue loading buffer already, which saved the step of mix loading buffer, and could indicate the electrophoresis progress after the PCR reaction. The migration speed of the blue dye in 1% TAE agarose gel is similar to a 500 bp double-stranded DNA fragment.
2×Taq PCR Super Fast Mix(with blue dye) is well-suited for super-fast PCR reactions, achieving an extension speed of 5-10 sec/kb, enabling the completion of the shortest PCR in as little as half an hour. It efficiently tackles complex templates with high GC content ( >60% ) and secondary structures, while also supporting large-scale gene detection. When working with short fragments or simple templates, such as plasmid templates, using an extension speed of 5 sec/kb can help reduce cycles and further shorten the PCR time, for long fragments ( ≥ 3 kb ) or complex templates producing low yields, an extension speed of 15-20 sec/kb or an increase in cycles may be required.
This product is suitable for large-scale genetic testing, semi-quantitative PCR experiments, and detection of trace DNA; it also facilitates rapid PCR amplification for cloning of expressed genes, analysis of intracellular gene point mutations (SNP), and other purposes.
Application Recommendations:
This product has a very strong amplification ability, and sometimes non-specific amplification may occur. The following methods can be used to reduce non-specific amplification bands.
(1) Increase the annealing temperature by 2-3 degrees;
(2) Reduce the mix volume, such as changing 10 μl to 8 μl;
(3) Reduce the number of cycles by 2 to reduce nonspecific amplification bands.
