Ac-RFAAKAA-COOH is a heptapeptide with one reactive Lys residue. Ac-RFAAKAA-COOH forms stable covalent adducts with isothiocyanates, resulting in peptide depletion over time. Ac-RFAAKAA-COOH acts as a model peptide in direct peptide reactivity assays, where its chemical reactivity is evaluated via fluorescamine-based free amine assays. Ac-RFAAKAA-COOH can be used in studies related to allergic contact dermatitis and skin sensitization [1] [2].
In Vitro, When used in a spectrophotometric depletion assay with a 30% depletion threshold (and a 10% depletion threshold for cysteine peptides), Ac-RFAAKAA-COOH can accurately predict the skin sensitization potential of test chemicals, with a sensitivity of 80.0%, specificity of 86.7%, and accuracy of 82.5%[2].
References:
[1]. Karlsson I, et al. Peptide Reactivity of Isothiocyanates--Implications for Skin Allergy. Sci Rep. 2016;6:21203. Published 2016 Feb 17.
[2]. Cho SA, et al. Method for detecting the reactivity of chemicals towards peptides as an alternative test method for assessing skin sensitization potential. Toxicol Lett. 2014;225(1):185-191.
In Vitro, When used in a spectrophotometric depletion assay with a 30% depletion threshold (and a 10% depletion threshold for cysteine peptides), Ac-RFAAKAA-COOH can accurately predict the skin sensitization potential of test chemicals, with a sensitivity of 80.0%, specificity of 86.7%, and accuracy of 82.5%[2].
References:
[1]. Karlsson I, et al. Peptide Reactivity of Isothiocyanates--Implications for Skin Allergy. Sci Rep. 2016;6:21203. Published 2016 Feb 17.
[2]. Cho SA, et al. Method for detecting the reactivity of chemicals towards peptides as an alternative test method for assessing skin sensitization potential. Toxicol Lett. 2014;225(1):185-191.