ACSF |
Catalog No.GC11850
One-Click Copy Product Info
|
replacement of CSF
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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ACSF is often used as replacement of CSF for perfusion of brain slices to preserve interneurons [1].
ACSF (artificial cerebrospinal fluid) is invented to reduce the incidence of cerebral edema and further suppress brain cell disorders. And ACSF is often used as an irrigation fluid or perfusion fluid in the field of neurosurgery, such as intracranial surgery, or when used as a replenishing fluid for lost cerebrospinal fluid. ACSF is also used to maintain pH balance and tissue oxygen delivery for research [2, 3].
ACSF is often used to maintain oxygen supply in the study of basic characteristics of neuronal function where acute in vitro brain slice models are required. When tested with neocortical mouse slices, carbogenated ACSF treatment could keep pH balance and tissue oxygen delivery; otherwise, seizure-like events were occurred in the slices with no-magnesium ACSF treatment [1]. In the study of targeted drugs for protect neuron, ACSF is often to treat the mice and worked as a control to calculate the drug efficiency [3]
It is also reported in the rat brain slice model, treated with ACSF, a replacement of human CSF, could powerfully boost spontaneous firing of CA1, CA3 and layer 5 pyramidal neurons [4].
References:
[1]. Voss, L.J., S.A. George, and J.W. Sleigh, Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. J Neurosci Methods, 2012. 204(2): p. 273-5.
[2]. Liu, D. and F. Bao, Hydrogen peroxide administered into the rat spinal cord at the level elevated by contusion spinal cord injury oxidizes proteins, DNA and membrane phospholipids, and induces cell death: Attenuation by a metalloporphyrin. Neuroscience, 2015. 285: p. 81-96.
[3]. Gruber, R.C., et al., Targeted GAS6 delivery to the CNS protects axons from damage during experimental autoimmune encephalomyelitis. J Neurosci, 2014. 34(49): p. 16320-35.
[4]. Bjorefeldt, A., et al., Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice. J Physiol, 2015. 593(1): p. 231-43.
| Cas No. | SDF | ||
| Formula | C20H20N2O2 | M.Wt | 320.38 |
| Solubility | Soluble to 5 mM in ethanol with gentle warming | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.1213 mL | 15.6065 mL | 31.2129 mL |
| 5 mM | 624.3 μL | 3.1213 mL | 6.2426 mL |
| 10 mM | 312.1 μL | 1.5606 mL | 3.1213 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)