C12FDG
Catalog No.: GC43021Purity:>98.00%Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
C12FDG is the fluorescent substrate of β-galactosidase.
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Cas No.: 138777-25-0
1mgIn Stock
$140.00
1
| Pack Size | Price | Availability | Quantity | Action |
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| 1mg | $140.00 | In Stock | 1 |
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Citations
3 publicationsPublications from researchers using this productDescription
C12FDG is the fluorescent substrate of β-galactosidase. By enzymolysis of β-galactosidase, fluorescein is released and its fluorescence can be used to quantify the activity of β-galactosidase. The excitation/emission maxima of fluorescein are 485/530 nm[1-3].
C12FDG(15 μM ; 2 h) staining can assess SA-β-Gal activity to detect senescence in THP-1 cells(15 μM ; 2 h) [4]and A172 cells(90 min; 33 µM) [5]. Live cell measurements of senescence-associated β-galactosidase (SA-β-Gal) activity using the fluorescent substrate C12FDG in combination with the determination of the total cell number using a DNA intercalating Hoechst dye opens the possibility to screen for senotherapeutic drugs [6].
References:
[1]. Zhang YZ, Naleway JJ, et,al. Detecting lacZ gene expression in living cells with new lipophilic, fluorogenic beta-galactosidase substrates. FASEB J. 1991 Dec;5(15):3108-13. doi: 10.1096/fasebj.5.15.1720751. PMID: 1720751.
[2]. Plovins A, Alvarez AM, et,al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41. doi: 10.1128/aem.60.12.4638-4641.1994. PMID: 7811104; PMCID: PMC202038.
[3]. Fuhrmann-Stroissnigg H, Santiago FE, et,al. SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs. J Vis Exp. 2019 Jun 28;(148). doi: 10.3791/58133. PMID: 31305507.
[4]. Díaz M, Pibuel M, et,al. 4-Methylumbelliferone induces antitumor effects independently of hyaluronan synthesis inhibition in human acute leukemia cell lines. Life Sci. 2021 Dec 15;287:120065. doi: 10.1016/j.lfs.2021.120065. Epub 2021 Oct 19. PMID: 34678263.
[5]. Beltzig L, Frumkina A, et,al. Cytotoxic, Genotoxic and Senolytic Potential of Native and Micellar Curcumin. Nutrients. 2021 Jul 13;13(7):2385. doi: 10.3390/nu13072385. PMID: 34371895; PMCID: PMC8308652.
[6].Waltz RA, Whitney KE, et,al. A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury-A Pilot Case Series. Life (Basel). 2023 Jul 15;13(7):1567. doi: 10.3390/life13071567. PMID: 37511942; PMCID: PMC10381817.
Protocol
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Protocol for Fluorescent aging associated β-galactosidase (SA-β-Gal) experiment using C12FDG [1]: |
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1. Cells were cultured with a density of 105 cells/mL. 2. The cells were washed once with 200 μL PBS, and then fixed with 100 μL fixing solution (2% formaldehyde /0.2% glutaraldehyde distilled water) at room temperature for 5 minutes. 3. The cells were washed with 200 μL PBS twice, stained with 100 μL 33 μM C12FDG (PBS, pH=6.0) for 10 min, and then 200 μL Hoechst solution (1 μg/mL Hoechst 33342) on PBS (pH 6.0) for 10 minutes. 4. These cells were imaged with a 20× objective lens and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), and monitored with emission filters of 460 nm and 535 nm, respectively. This C12FDG protocol only provides a guideline, and should be modified according to your specific needs. |
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Protocol for Measurement of Senescence-Associated β-galactosidase activity using C12FDG [2]: |
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The fluorogenic cell permeable substrate C12FDG was used to determine the senescence-associated β-galactosidase activity (SA-β-gal) in treated Endothelial cells by flow cytometry.
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References: [1]. Udono M, Kadooka K, et,al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8. doi: 10.1016/j.ymeth.2012.02.012. Epub 2012 Mar 3. PMID: 22406489. [2]. Qureshi AW, Altamimy R, et,al. Ageing enhances the shedding of splenocyte microvesicles with endothelial pro-senescent effect that is prevented by a short-term intake of omega-3 PUFA EPA:DHA 6:1. Biochem Pharmacol. 2020 Mar;173:113734. doi: 10.1016/j.bcp.2019.113734. Epub 2019 Dec 5. PMID: 31811867. |
Product Documents
Purity:>98.00%Appearance:A solid
Biological Data
1 / 1
Chemical Properties
CAS No.
138777-25-0
Synonyms
5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
Chemical Name
N-[3',6'-bis(β-D-galactopyranosyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]-dodecanamide
SMILES
O[C@@H]([C@@H](O)[C@@H](CO)O1)[C@@H](O)[C@@H]1OC2=CC=C(C3(C4=CC=C(NC(CCCCCCCCCCC)=O)C=C4C(O3)=O)C(C=CC(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)=C6)=C6O7)C7=C2
Molecular Formula
C44H55NO16
Molecular Weight
853.9
Solubility
DMSO : 50 mg/mL (58.55 mM; Need ultrasonic)
Storage
Store at -20°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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