10,12-Tricosadiynoic acid |
رقم الكتالوجGC38881
One-Click Copy Product Info
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10،12-Tricosadiynoic acid هو مثبط عالي التحديد وانتقائي وعالي التقارب ونشط عن طريق الفم من مثبط أسيل- CoA أوكسيديز -1 (ACOX1)
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 66990-30-5
Sample solution is provided at 25 µL, 10mM.
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10,12-Tricosadiynoic acid is a highly specific, selective, and orally active inhibitor of acyl-CoA oxidase-1 (ACOX1)[1]. ACOX1 functions in peroxisomes, primarily responsible for fatty acid metabolism, especially the β-oxidation of long-chain fatty acids, and plays a crucial role in maintaining cellular health and lipid homeostasis[2]. 10,12-Tricosadiynoic acid can be used to treat metabolic diseases (such as those induced by obesity) by improving lipid and reactive oxygen species (ROS) metabolism, and serves as a click chemistry reagent due to its alkyne group[3,4].
In vitro, treatment of HL-7702 and HEP-G2 cells with 10,12-Tricosadiynoic acid (500nM) together with GW4064 for 48h effectively reversed the GW4064-induced upregulation of ACOX1 protein and mRNA expression[5]. Treatment of SiHa and HCT116 cancer cells cultured in an acidic environment (pH 6.5) with 10,12-Tricosadiynoic acid (60μM) combined with docosahexaenoic acid (DHA, 50μM) for 24h significantly reduced cell viability[6].
In vivo, oral administration of 10,12-Tricosadiynoic acid (100μg/kg/day) to Wistar rats fed a high-fat diet (HFD) for 8 weeks significantly reduced body weight gain, decreased liver weight and triglyceride content, and alleviated hepatic steatosis[1]. Oral administration of 10,12-Tricosadiynoic acid (100mg/kg/day) for 4 weeks to diabetic mice chronically fed high erucic acid rapeseed oil (HRO) significantly inhibited hepatic peroxisomal β-oxidation and cholesterol synthesis, and reduced plasma very low-density lipoprotein cholesterol levels[7].
References:
[1] ZENG J, DENG S, WANG Y, et al. Specific inhibition of Acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet[J]. Journal of Biological Chemistry, 2017, 292(9): 3800-3809.
[2] LU D, HE A, TAN M, et al. Liver ACOX1 regulates levels of circulating lipids that promote metabolic health through adipose remodeling[J]. Nature Communications, 2024, 15(1): 4214.
[3] LIU Y, HAN S L, LUO Y, et al. Impaired peroxisomal fat oxidation induces hepatic lipid accumulation and oxidative damage in Nile tilapia[J]. Fish Physiology and Biochemistry, 2020, 46(4): 1229-1242.
[4] CHEN X, ZHOU G, PENG X, et al. Biosensors and chemosensors based on the optical responses of polydiacetylenes[J]. Chemical Society Reviews, 2012, 41(13): 4610-4630.
[5] YANG Y, YUAN W, HE K, et al. Inhibition of ACOX1 enhances the therapeutic efficacy of obeticholic acid in treating non-alcoholic fatty liver disease and mitigates its lipotoxicity[J]. Frontiers in Pharmacology, 2024, 15: 1366479.
[6] IBANEZ S, GIOLITO M V, AL-SIYABI S, et al. Acidosis forces fatty acid uptake and metabolism in cancer cells regardless of genotype[J]. Advanced Science, 2025, 12(40): e05436.
[7] ZHANG X, WANG Y, YAO H, et al. Peroxisomal β-oxidation stimulates cholesterol biosynthesis in the liver in diabetic mice[J]. Journal of Biological Chemistry, 2022, 298(2): 101572.
| Cell experiment [1]: | |
Cell lines | HL-7702 and HEP-G2 cells |
Preparation Method | The cells were treated with 65nM and 500nM concentrations of GW4064 and 10,12-Tricosadiynoic acid, respectively. After 48h of drug incubation, the cells were harvested to extract total RNA and protein for Real-Time Quantitative PCR and Western blotting, respectively. |
Reaction Conditions | 500nM; 48h |
Applications | Treatment with 10,12-Tricosadiynoic acid effectively reversed the upregulation of ACOX1 protein and mRNA expression induced by GW4064. |
| Animal experiment [2]: | |
Animal models | HFD-fed Wistar rats |
Preparation Method | HFD-fed Wistar rats were treated with 10,12-Tricosadiynoic acid (100μg/kg/day) for 8 weeks, with body weight measured every two weeks. On day 57, all rats were euthanized, and their livers were removed, weighed, and examined for hepatic steatosis using an optical microscope. |
Dosage form | 100μg/kg/day; 8 weeks; p.o. |
Applications | Treatment with 10,12-Tricosadiynoic acid (100μg/kg/day) significantly reduced weight gain and liver weight, and alleviated hepatic steatosis. |
References: | |
| Cas No. | 66990-30-5 | SDF | |
| Canonical SMILES | CCCCCCCCCCC#CC#CCCCCCCCCC(O)=O | ||
| Formula | C23H38O2 | M.Wt | 346.55 |
| الذوبان | DMSO : 100 mg/mL (288.56 mM） | Storage | Store at 2-8°C,protect from light, stored under argon |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8856 mL | 14.4279 mL | 28.8559 mL |
| 5 mM | 577.1 μL | 2.8856 mL | 5.7712 mL |
| 10 mM | 288.6 μL | 1.4428 mL | 2.8856 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)