Aldehyde Reactive Probe (trifluoroacetate salt) (Synonyms: ARP, O(Biotinylcarbazoylmethyl) Hydroxylamine) |
رقم الكتالوجGC42765
One-Click Copy Product Info
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يتعرض الحمض النووي باستمرار للتلف بسبب عوامل داخلية وبيئية مما يؤدي إلى تكون مواقع نقصان أساسية (AP) التي تعطِّل عملية توليد الحمض النووي.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 627090-10-2
Sample solution is provided at 25 µL, 10mM.
DNA is continually damaged by endogenous and environmental agents leading to the formation of abasic (apurinic/apyrimidinic, AP) sites that are disruptive to DNA synthesis. Aldehyde Reactive Probe (ARP) is a biotinylated reagent for the detection and quantification of AP sites in damaged DNA. ARP reacts with aldehyde groups formed when reactive oxygen species depurinate DNA, resulting in covalent linkage of biotin to these AP sites. The biotin-tagged DNA can then be detected using common avidin-conjugated reporters such as avidin-HRP. The ARP method is highly sensitive, enabling detection of 2.4 AP sites per 1x107 nucleotides of DNA.
| Cas No. | 627090-10-2 | SDF | |
| المرادفات | ARP, O(Biotinylcarbazoylmethyl) Hydroxylamine | ||
| Canonical SMILES | O=C1N([H])[C@@]2([H])[C@@](CS[C@H]2CCCCC(N([H])N([H])C(CON)=O)=O)([H])N1[H].FC(F)(C(O)=O)F | ||
| Formula | C12H21N5O4S•CF3COOH | M.Wt | 445.4 |
| الذوبان | DMF: 20 mg/ml DMSO: 10 mg/ml PBS (pH 7.2): 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2452 mL | 11.2259 mL | 22.4517 mL |
| 5 mM | 449 μL | 2.2452 mL | 4.4903 mL |
| 10 mM | 224.5 μL | 1.1226 mL | 2.2452 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)