Angiotensin II
Catalog No.: GP10023Purity:>99.50%
محفز عند مستقبلات AT1 وAT2
المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.
Cas No.: 4474-91-3
1mgIn Stock
$12.00
1
5mgIn Stock
$26.00
1
10mgIn Stock
$41.00
1
25mgIn Stock
$68.00
1
50mgIn Stock
$95.00
1
100mgIn Stock
$140.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 1mg | $12.00 | In Stock | 1 | |
| 5mg | $26.00 | In Stock | 1 | |
| 10mg | $41.00 | In Stock | 1 | |
| 25mg | $68.00 | In Stock | 1 | |
| 50mg | $95.00 | In Stock | 1 | |
| 100mg | $140.00 | In Stock | 1 |
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المراجع (Citations)
16 publicationsمنشورات الباحثين الذين استخدموا هذا المنتجالوصف (Description)
Angiotensin II is a major effector peptide of the renin-angiotensin system (RAS). Angiotensin II binds to AT1R to mediate vasoconstriction and promote cardiac hypertrophy and fibrosis. Angiotensin II activates NADPH oxidase to produce ROS and the NF-κB pathway, exacerbating inflammation and oxidative stress. Angiotensin II can be used in research on hypertension, heart failure, and related cardiovascular diseases[1-4].
In vitro, Angiotensin II (1μM) treated INS-1 rat insulinoma cells for 24h. Angiotensin II reduced INS-1 cell viability, promoted apoptosis, and upregulated TXNIP expression and NLRP3 inflammasome components (NLRP3, ASC, caspase-1 p10)[5]. Amyloid-β(1-42) (0.5μM) pretreated PC12 cells for 24h, then Angiotensin II (5, 50, 500nM) was added for 24h. Angiotensin II dose-dependently upregulated LC3-II/I and Atg3, Atg7, Beclin1 expression, and downregulated p62 and Bcl-2. Angiotensin II significantly induced apoptosis[6].
In vivo, Angiotensin II (1.0μg/kg/min) was subcutaneously infused via osmotic pump into C57BL/6 mice and Ins+/Akita mice for 2 weeks. Angiotensin II elevated systolic blood pressure in mice and reduced islet endothelial volume. Angiotensin II worsened hyperglycemia after oral glucose load and decreased glucose-stimulated insulin secretion under in situ pancreatic perfusion[7]. Angiotensin II (1.44mg/kg) was subcutaneously infused via osmotic pump into arthritis model C57BL/6 mice for 4 weeks. Angiotensin II aggravated arthritis in mice and induced a decrease in talar bone volume[8].
References:
[1] Bumpus FM, Pucell AG, Daud AI, Husain A. Angiotensin II: an intraovarian regulatory peptide. Am J Med Sci. 1988 Apr;295(4):406-8.
[2] Kinugawa S. Angiotensin II and skeletal muscle abnormalities. Exp Physiol. 2017 Jun 1;102(6):614-615.
[3] Ferrario CM. Angiotensin I, angiotensin II and their biologically active peptides. J Hypertens. 2002 May;20(5):805-7.
[4] Peach MJ, Dostal DE. The angiotensin II receptor and the actions of angiotensin II. J Cardiovasc Pharmacol. 1990;16 Suppl 4:S25-30.
[5] Wang J, Feng Y, Huo H, et al. NLRP3 inflammasome mediates angiotensin II-induced islet β cell apoptosis. Acta Biochim Biophys Sin. 2019 May;51(5):501-508.
[6] Tian M, Lin X, Wu L, et al. Angiotensin II Triggers Autophagy and Apoptosis in PC12 Cell Line: An in vitro Alzheimer's Disease Model. Brain Res. 2019 Sep;1718:46-52.
[7] Ihoriya C, Satoh M, Kuwabara A, et al. Angiotensin II regulates islet microcirculation and insulin secretion in mice. Microcirculation. 2014 Feb;21(2):112-123.
[8] Akagi T, Mukai T, Mito T, et al. Effect of Angiotensin II on Bone Erosion and Systemic Bone Loss in Mice with Tumor Necrosis Factor-Mediated Arthritis. Int J Mol Sci. 2020 Jun;21(11):4145.
البروتوكول (Protocol)
| Cell experiment [1]: | |
|
Cell lines |
INS-1 cells (rat insulinoma cell line) |
|
Preparation Method |
INS-1 cells were cultured in RPMI 1640 medium supplemented with 12% fetal bovine serum, penicillin, and streptomycin at 37°C, 5% CO₂. Cells were treated with Angiotensin II at indicated concentrations and times. |
|
Reaction Conditions |
1μM; 24h |
|
Applications |
Angiotensin II reduced INS-1 cell viability, increased apoptosis rate and cleaved caspase-3/caspase-3 ratio, upregulated TXNIP mRNA and protein expression, upregulated NLRP3, ASC, and caspase-1 p10 protein expression, and increased IL-1β secretion in the supernatant. |
| Animal experiment [2]: | |
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Animal models |
Male C57BL/6 mice and male Ins+/Akita mice (6 weeks old) |
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Preparation Method |
C57BL/6 and Ins+/Akita mice were subcutaneously infused with Angiotensin II (1.0μg/kg/min) via osmotic minipump for 2 weeks; systolic blood pressure, oral glucose tolerance, and in situ pancreatic perfusion were assessed, and pancreas tissues were collected for histological analysis. |
|
Dosage form |
1.0μg/kg/min; s.c. via osmotic minipump; 2 weeks |
|
Applications |
Angiotensin II elevated systolic blood pressure, reduced islet endothelial volume, exacerbated hyperglycemia on oral glucose tolerance test, and decreased glucose-stimulated insulin secretion in both first and second phases during in situ pancreatic perfusion. |
|
References: [1] Wang J, Feng Y, Huo H, et al. NLRP3 inflammasome mediates angiotensin II-induced islet β cell apoptosis. Acta Biochim Biophys Sin. 2019 May;51(5):501-508. [2] Ihoriya C, Satoh M, Kuwabara A, et al. Angiotensin II regulates islet microcirculation and insulin secretion in mice. Microcirculation. 2014 Feb;21(2):112-123. |
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وثائق المنتج (Product Documents)
Purity:>99.50%Appearance:A solid
الخصائص الكيميائية (Chemical Properties)
رقم CAS
4474-91-3
الاسم الكيميائي
Angiotensin II
SMILES
CCC(C)C(C(=O)NC(CC1=CN=CN1)C(=O)N2CCCC2C(=O)NC(CC3=CC=CC=C3)C(=O)O)NC(=O)C(CC4=CC=C(C=C4)O)NC(=O)C(C(C)C)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC(=O)O)N.CC(=O)O
الصيغة الجزيئية
C50H71N13O12
الوزن الجزيئي
1046.2
الذوبانية
≥100.2mg/mL in Water
التخزين
Store at -20°C, protect from light, stored under nitrogen
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.
الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية
g/mol
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