8-hydroxybergapten is O-methylated by cell-free extracts of Ruta cells to isopimpinellin, in reactions mediated by discrete O-methyltransferases. 8-hydroxybergapten has excellent anti-wrinkle effect[1][2].
[1]. Brown SA, et al. The biosynthesis of isopimpinellin. Can J Biochem. 1977 Jul;55(7):686-92. [2]. Mu-Hyun Jin, et al. Composition for promoting synthesis of collagen, and composition for external preparation for skin comprising the same.