Trehalose (hydrate) (Synonyms: D-(+)-Trehalose) |
رقم الكتالوجGC45075
One-Click Copy Product Info
|
Trehalose (hydrate) is a natural, safe, non-specific disaccharide osmoprotectant.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6138-23-4
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Trehalose (hydrate) is a natural, safe, non-specific disaccharide osmoprotectant[1]. Trehalose (hydrate) exerts anti-desiccation, anti-freezing, and anti-oxidative effects by stabilizing protein and membrane structures and maintaining osmotic pressure[2]. Trehalose (hydrate) is widely used in studies of metabolic diseases, neurodegenerative disorders, and food–drug delivery systems[3][4].
In vitro, Trehalose (hydrate) (100mM; 72h) significantly increases the viability of H9c2 cardiomyocytes exposed to high glucose (33mM), decreases the Bax/Bcl-2 ratio and cleaved-caspase-3 protein levels, suppresses NALP3/caspase-1/IL-1β/IL-18-mediated pyroptosis, restores the expression of Beclin-1 and Atg5 proteins as well as the LC3-II/I ratio, and reverses high-glucose–induced autophagic impairment[5].
In vivo, Trehalose (hydrate) (4% w/v in drinking water; 14 days; ad libitum) fully reversed the memory deficit induced by bilateral i.c.v. Aβ25–35 injection, restoring step-through latency in the passive avoidance test to control levels in APP/PS1 mice, while significantly reducing hippocampal CA1 Aβ fluorescence intensity and IBA1-positive microglial density, and restoring neuronal density to control values as shown by Nissl staining[6]. Trehalose (hydrate) (2% w/v in drinking water; ad libitum from 64 days of age until death) significantly delayed disease onset and extended mice lifespan, doubled motor neuron survival in the L4–5 spinal cord, and reduced spinal insoluble SOD1 aggregates in SOD1G93A mice[7].
References:
[1] Megarry AJ, Booth J, Burley J. Amorphous trehalose dihydrate by cryogenic milling. Carbohydr Res. 2011;346(8):1061-1064.
[2] Chen A, Tapia H, Goddard JM, Gibney PA. Trehalose and its applications in the food industry. Compr Rev Food Sci Food Saf. 2022;21(6):5004-5037.
[3] Sharma E, Shruti PS, Singh S, et al. Trehalose and its Diverse Biological Potential. Curr Protein Pept Sci. 2023;24(6):503-517.
[4] Sevriev B, Dimitrova S, Kehayova G, Dragomanova S. Trehalose: Neuroprotective Effects and Mechanisms-An Updated Review. NeuroSci. 2024;5(4):429-444.
[5] Liu Y, Wu S, Zhao Q, et al. Trehalose Ameliorates Diabetic Cardiomyopathy: Role of the PK2/PKR Pathway. Oxid Med Cell Longev. 2021;2021:6779559.
[6] Pupyshev AB, Akopyan AA, Tenditnik MV, et al. Alimentary Treatment with Trehalose in a Pharmacological Model of Alzheimer's Disease in Mice: Effects of Different Dosages and Treatment Regimens. Pharmaceutics. 2024;16(6):813.
[7] Zhang X, Chen S, Song L, et al. MTOR-independent, autophagic enhancer trehalose prolongs motor neuron survival and ameliorates the autophagic flux defect in a mouse model of amyotrophic lateral sclerosis. Autophagy. 2014;10(4):588-602.
| Cell experiment [1]: | |
Cell lines | Rat H9c2 cardiomyocytes |
Preparation Method | Rat H9c2 cardiomyocytes were cultured in DMEM low-sugar medium supplemented with 10% FBS and a 1% penicillin-streptomycin solution at 37°C and 5% CO2 in a humidified environment. Cells were incubated with 33mM high glucose for 72h with or without different concentrations of Trehalose (hydrate) (100mM). The MTT assay was performed according to the manufacturer’s instructions. |
Reaction Conditions | 100mM; 72h |
Applications | Trehalose (hydrate) significantly increases the viability of H9c2 cardiomyocytes exposed to high glucose. |
| Animal experiment [2]: | |
Animal models | Male SOD1G93A mice |
Preparation Method | Male SOD1G93A mice were randomly divided into 3 groups (Tg-NT, Tg-Suc, Tg-Tre) with 21 mice in each group and these mice were housed under the conditions of constant temperature and controlled lighting (light/dark period 12/12h). For Tg-Tre mice, 2% Trehalose (hydrate) containing water was given to SOD1G93A mice through ad libitum consumption, starting at 64d of age and continuing until the day the mice died. For Tg-Suc mice, 2% sucrose-containing water was given to mice as a vehicle control. For Tg-NT mice, no treatment was given to SOD1G93A mice. In addition, 15 of the age-matched WT littermates were used as controls. Rotarod performance was measured in SOD1G93A mice starting at 80d of age. SOD1G93A mice developed complete paralysis roughly at around 126d of age. For life-span analysis, the date of “death” (or terminal impairment) was defined as the day when the mice could not right itself within 30s after being placed on its back. |
Dosage form | 2% w/v in drinking water; ad libitum from 64 days of age until death |
Applications | Trehalose (hydrate) significantly delayed disease onset and extended mice lifespan in SOD1G93A mice. |
References: | |
| Cas No. | 6138-23-4 | SDF | |
| المرادفات | D-(+)-Trehalose | ||
| Canonical SMILES | OC[C@@H](O1)[C@@H](O)[C@H](O)[C@@H](O)[C@@]1([H])O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O2.O.O | ||
| Formula | C12H22O11•2H2O | M.Wt | 378.3 |
| الذوبان | Water : 150 mg/mL (396.48 mM) | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.6434 mL | 13.217 mL | 26.434 mL |
| 5 mM | 528.7 μL | 2.6434 mL | 5.2868 mL |
| 10 mM | 264.3 μL | 1.3217 mL | 2.6434 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)