الصفحة الرئيسية>>Signaling Pathways>> Stem Cell>> Oct3/4>>OAC2

OAC2

رقم الكتالوجGC17327

OAC2 هو مركب منشط Oct4 ينشط التعبير من خلال محفز الجينات Oct4

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OAC2 التركيب الكيميائي

Cas No.: 6019-39-2

الحجم السعر المخزون الكميّة
10mg
39٫00
متوفر
50mg
164٫00
متوفر
100mg
281٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Description of OAC2

OAC2 is identified as an activator of octamer-binding transcription factor 4.

Octamer-binding transcription factor 4 (Oct4) has been found to be a key regulator of embryonic stem cell (ESC) pluripotency and cirtical to the reprogramming process.

In vitro: OAC2 was found to be able to activate both Oct4 and Nanog reporters to a similar extent as OAC1, which was the OAC analog showing greatest activating effects on both Oct4 and Nanog promoter-driven luciferase reporter genes. Moreover, in order to examine the developmental potential of 4F+OAC1- and 4F+OAC2-iPSCs, the authors differentiated such cells in vitro by using a standard embryoid body differentiation method. The immunostaining results showed that both the 4F+ OAC1-iPSCs and 4F+OAC2-iPSCs were able to effectively differentiate into characteristic smooth muscle actin (SMA)+ mesodermal cells, FoxA2+ endoderm cells, and Tuj1+ ectoderm cells as well [1].

In vivo: In animal to test the in vivo pluripotency of the 4F+ OAC2-induced iPSCs, the 4F+OAC2-iPSCs were transplanted the into immunodeficient Nude mice. Four to 6 weeks after transplantation, the 4F+OAC2-iPSCs could generate typical teratomas containing derivative of all three germ layers effectively, such as blood of mesoderm, intestinal epithelia of endoderm, as well as epidermis of ectoderm [1].

Clinical trial: Up to now, OAC2 is still in the preclinical development stage.

Reference:
[1] Li W,Tian E,Chen ZX,Sun G,Ye P,Yang S,Lu D,Xie J,Ho TV,Tsark WM,Wang C,Horne DA,Riggs AD,Yip ML,Shi Y.  Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A.2012 Dec 18;109(51):20853-8.

Protocol of OAC2

Cell experiment:

The Oct4-luc or Nanog-luc cells are treated with compound OAC1 or its structural analogs OAC2, OAC3 at 1 μM concentration or at indicated concentrations. Other compounds used include 2 μM BIO, 2 μM BIX, 2 μM 5'-azacytidine, 25 μg/mL Vitamin C, 10 nM Am580, 5 μM tranylcypromine, and 0.5 mM valporic acid. Luciferase reporter assays are performed 24 h after compound treatment or at indicated time points. For Topflash reporter assays, 0.2 μg β-catenin–responsive Topflash reporter gene plasmid is introduced into CV1 cells using trasfection. Compounds are added 6 h after transfection. Luciferase activity is measured 48 h after compound treatment using the Glo Luciferase Assay System[2].

References:

[1]. Okuyama T, et al. Structural and mechanistic insights into nuclear transport and delivery of the critical pluripotency factor Oct4 to DNA. J Biomol Struct Dyn. 2017 Feb 6:1-50
[2]. Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.

Chemical Properties of OAC2

Cas No. 6019-39-2 SDF
Chemical Name N-(1H-indol-5-yl)benzamide
Canonical SMILES O=C(NC1=CC=C2C(C=CN2)=C1)C3=CC=CC=C3
Formula C15H12N2O M.Wt 236.27
الذوبان ≥ 23.6mg/mL in DMSO, ≥ 24.4 mg/mL in EtOH with ultrasonic Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of OAC2

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 4.2324 mL 21.1622 mL 42.3245 mL
5 mM 0.8465 mL 4.2324 mL 8.4649 mL
10 mM 0.4232 mL 2.1162 mL 4.2324 mL
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In vivo Formulation Calculator (Clear solution) of OAC2

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 6 reference(s) in Google Scholar.)

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