Oleic acid (9-cis-Octadecenoic acid), a major monounsaturated fatty acid (MUFA) in the circulatory system, is an energy molecule and an element of cell membranes^[^1]. Oleic acid diminishes the expression of cholesterol transport-related proteins, decreases cholesterol absorption, and reduces the oxidation of low-density lipoprotein (LDL), preventing atherosclerosis^[2]. Oleic acid has been widely used in diabetes research to inhibit endoplasmic reticulum (ER) stress, prevent the weakening of the insulin signaling pathway, and improve the survival of beta cells^[3].

In vitro, Oleic acid treatment for 72 hours significantly reduced the proliferation of KLE, Hec-1B and ECC-1 cells, with IC₅₀ values of 445.6μM, 382.8μM and 369.8μM, respectively^[4]. Treatment with 25μM Oleic acid for 48 hours inhibited stearic acid-induced growth inhibition and pro-inflammatory responses in human aortic endothelial cells (HAECs)^[5]. Treatment with 1.32mM oleic acid for 24 hours induced lipid accumulation and increased the expression of PPARγ and SREBP-1 in HepG2 cells^[6].

In vivo, Oleic acid treatment via a single intratracheal injection at a dose of 10mg/kg within 24 hours elicited pulmonary inflammation in Swiss Webster mice, promoting the activation of lung white blood cells and the production of inflammatory mediators^[7]. Oral administration of Oleic acid (160mg/kg) daily for 14 days improved the Angiotensin II-induced cardiac remodeling, and inhibited cardiomyocyte hypertrophy and collagen deposition in mice^[8].

References:

[1] Karacor K, Cam M. Effects of oleic acid[J]. Medical Science and Discovery, 2015, 2(1): 125-32.

[2] Santa-María C, López-Enríquez S, Montserrat-de la Paz S, et al. Update on anti-inflammatory molecular mechanisms induced by oleic acid[J]. Nutrients, 2023, 15(1): 224.

[3] Palomer X, Pizarro-Delgado J, Barroso E, et al. Palmitic and oleic acid: the yin and yang of fatty acids in type 2 diabetes mellitus[J]. Trends in Endocrinology & Metabolism, 2018, 29(3): 178-190.

[4] Deng B, Kong W, Suo H, et al. Oleic acid exhibits anti-proliferative and anti-invasive activities via the PTEN/AKT/mTOR pathway in endometrial cancer[J]. Cancers, 2023, 15(22): 5407.

[5] Harvey K A, Walker C L, Xu Z, et al. Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells[J]. Journal of lipid research, 2010, 51(12): 3470-3480.

[6] Ricchi M, Odoardi M R, Carulli L, et al. Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes[J]. Journal of gastroenterology and hepatology, 2009, 24(5): 830-840.

[7] Gonçalves-de-Albuquerque C F, Silva A R, Burth P, et al. Oleic acid induces lung injury in mice through activation of the ERK pathway[J]. Mediators of Inflammation, 2012, 2012(1): 956509.

[8] Liu T, Wen H, Li H, et al. Oleic acid attenuates Ang II (angiotensin II)-induced cardiac remodeling by inhibiting fgf23 (fibroblast growth factor 23) expression in mice[J]. Hypertension, 2020, 75(3): 680-692.

البروتوكول (Protocol)

Cell experiment [1]:
Cell lines	Hec-1B cells
Preparation Method	Hec-1B cells were cultured in McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS), 100U/ml of penicillin and 100µg/ml of streptomycin at 37°C in an incubator with 5% CO₂. Cells (4000/well) were seeded in 96-well plates and cultured at 37°C in 5% CO₂ overnight and then treated with 0.1, 1, 10, 50, 100, 250, and 500µM Oleic acid for 72h, cell proliferation was assessed.
Reaction Conditions	0.1, 1, 10, 50, 100, 250, and 500µM; 72h
Applications	Oleic acid treatment reduced cell proliferation of Hec-1B cells in a dose-dependent manner.
Animal experiment [2]:
Animal models	Male C57BL/6 mice
Preparation Method	Male C57BL/6 mice (10-week-old) were housed in a specific-pathogen-free environment under a 12h/12h light-dark cycle and fed rodent diet ad libitum. After mice orally received Oleic acid (160mg/kg/day) or saline for 14 days, cardiac remodeling was induced by chronic infusion of Ang II at a dose of 1000ng/kg per minute for 2 weeks with a pump. Mice were anesthetized with 3.0% isoflurane mixed with oxygen (100%; airflow velocity: 1L/minute) was used to maintain anesthesia. An incision was made in the skin on the back of the mouse, and the pump was implanted into the subcutaneous tissue. After being sutured and regaining consciousness, the mice were returned to their original cages and fed individually until the end of the experiment. Heart was excised and prepared for histology.
Dosage form	160mg/kg/day; 2 weeks; p.o.
Applications	Oleic acid treatment attenuated Angiotensin II-induced cardiac remodeling and reduced collagen deposition in the hearts of mice.
References: [1] Deng B, Kong W, Suo H, et al. Oleic acid exhibits anti-proliferative and anti-invasive activities via the PTEN/AKT/mTOR pathway in endometrial cancer[J]. Cancers, 2023, 15(22): 5407. [2] Liu T, Wen H, Li H, et al. Oleic acid attenuates Ang II (angiotensin II)-induced cardiac remodeling by inhibiting fgf23 (fibroblast growth factor 23) expression in mice[J]. Hypertension, 2020, 75(3): 680-692.

وثائق المنتج (Product Documents)

Batch:Purity:>98.00%Appearance:A liquid

COA SDS Data Sheet

البيانات البيولوجية (Biological Data)

1 / 1

الخصائص الكيميائية (Chemical Properties)

رقم CAS

112-80-1

المرادفات

9Z-Octadecenoic Acid, C18:1(9Z), cis-9-Octadecenoic acid

SMILES

CCCCCCCC/C=C\CCCCCCCC(O)=O

الصيغة الجزيئية

C₁₈H₃₄O₂

الوزن الجزيئي

282.46

الذوبانية

DMSO : ≥ 62.5 mg/mL (221.27 mM)

التخزين

Store at 2-8°C

شروط الشحن

تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.

الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية

الكتلة

الحجم

التركيز

الوزن الجزيئي

g/mol

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