RFRP-3 (mouse)

RFRP-3 (mouse) is a functional ortholog of avian gonadotropin inhibitory hormone (GnIH), binding to GPR147. RFRP-3 (mouse) reduces Progesterone synthesis by inhibiting FSHR and key enzymes involved in steroidogenesis (P450scc, 3β-HSD, StAR). RFRP-3 (mouse) induces Apoptosis (increase of p53). RFRP-3 (mouse) also suppresses the ERK signaling pathway. RFRP-3 (mouse) can be used for research of follicular development ^[1].

In Vitro, RFRP-3 (mouse) (10-1000 ng/mL, 24 h) significantly reduces Progesterone secretion but not Estradiol secretion in the ovarian granulosa cells of mice^[1]. RFRP-3 (mouse) (10-1000 ng/mL, 24 h) inhibits steroidogenesis and FSHR (inhibition of p450scc, 3β-HSD, StAR, and FSHR) in the ovarian granulosa cells of mice^[1]. RFRP-3 (mouse) (10-1000 ng/mL, 24 h) induces apoptosis, with lowering Bcl-2 protein expression and increasing p53 protein expression at the medium dose^[1]. RFRP-3 (mouse) (10-1000 ng/mL, 24 h) suppresses ERK signaling pathway (p-ERK1/2 protein) in the ovarian granulosa cells of mice^[1].

References:
[1]. Guo X, et al. Rfamide-related peptide-3(RFRP-3) receptor gene is expressed in mouse ovarian granulosa cells: Potential role of RFRP-3 in steroidogenesis and apoptosis. Steroids. 2024 Feb;202:109349.