WYneC is a chemoselective probe for the detection of sulfenylated proteins in live cells.1,2 It is selective for a sulfenylated dipeptide over sulfinic acid- or sulfonic acid-containing dipeptides, reduced glutathione (GSH), glutathione disulfide (GSSG), and S-nitrosoglutathione (GSNO), as well as cysteine sulfinic acid and glutathione sulfonic acid, in cell-free assays.1 WYneC also selectively binds to C64,82S glutathione peroxidase 3 (GPX3) containing a sulfenic acid at Cys36 over C64,82S GPX3 containing a reduced thiol, sulfinic acid at Cys36, or an intramolecular disulfide bond in cell-free assays. It binds to sulfenylated proteins in live cells and can then be conjugated to azide-containing fluorescent probes for detection.
References:
[1]. Shi, Y., Fu, L., Yang, J., et al.Wittig reagents for chemoselective sulfenic acid ligation enables global site stoichiometry analysis and redox-controlled mitochondrial targetingNat. Chem.13(11)1140-1150(2021).
[2]. He, J.-X., Fei, Z.-C., Fu, L., et al.A modification-centric assessment tool for the performance of chemoproteomic probesNat. Chem. Biol.18(8)904-912(2022).