Biotin-PEG2-alkyne is a click chemistry PEG linker based on copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), as well as a biotinylation reagent. In solution-phase and click labeling systems, Biotin-PEG2-alkyne efficiently conjugates biotin groups to azide-functionalized streptavidin amplicons. Biotin-PEG2-alkyne is also widely applicable in fixed cells and mouse intestinal tissue sections to achieve specific biotinylation of various azide-labeled biomolecules (such as proteins, lipids, DNA, and Afatinib ), thereby effectively promoting signal amplification and precise detection of targets [1].
In Vitro, The biotin moiety in Biotin-PEG2-alkyne renders it suitable for affinity-based applications such as pull-down assays, while the terminal alkyne is used for copper (I)-catalyzed click chemistry with azides.
References:
[1]. Bai J, et al. Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification. RSC Chem Biol. 2021;2(3):906-916. Published 2021 Mar 20.
In Vitro, The biotin moiety in Biotin-PEG2-alkyne renders it suitable for affinity-based applications such as pull-down assays, while the terminal alkyne is used for copper (I)-catalyzed click chemistry with azides.
References:
[1]. Bai J, et al. Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification. RSC Chem Biol. 2021;2(3):906-916. Published 2021 Mar 20.