GLPBio

Coronaridine

Catalog No.: GC64816Purity:>98.00%
COASDSData Sheet
Coronaridine is an inhibitor of TCF/β-catenin, with an IC50 value of 5.8μM.

Products are for research use only. Not for human use. We do not sell to patients.

Coronaridine
Cas No.: 467-77-6
1mgIn Stock
$216.00
1
5mgIn Stock
$540.00
1
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Description

Coronaridine is an inhibitor of TCF/β-catenin, with an IC50 value of 5.8μM[1]. Coronaridine has been widely used in neurological function studies and in development of congeners[2].

In vitro, Coronaridine showed potent cytotoxic activity in the laryngeal carcinoma cell line Hep-2, with an IC50 value of 54.47µg/mL) after 24h treatment[3]. Coronaridine treatment (10μg/ml; 24h) exhibited potent antileishmanial activity, suppressing promastigote and amastigote growth[4].

In vivo, Coronaridine treatment (72mg/kg; p.o.) for 25 minutes produced a sedative effect and reduced spontaneous activity in Male CD-1 albino mice [5].

References:
[1] Ohishi K, Toume K, Arai M A, et al. Coronaridine, an iboga type alkaloid from Tabernaemontana divaricata, inhibits the Wnt signaling pathway by decreasing β-catenin mRNA expression[J]. Bioorganic & medicinal chemistry letters, 2015, 25(18): 3937-3940.
[2] Arias H R, Tae H S, Micheli L, et al. Coronaridine congeners decrease neuropathic pain in mice and inhibit α9α10 nicotinic acetylcholine receptors and CaV2. 2 channels[J]. Neuropharmacology, 2020, 175: 108194.
[3] Rizo W F, Ferreira L E, Colnaghi V, et al. Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A. DC in a human laryngeal epithelial carcinoma cell line (Hep-2)[J]. Genetics and molecular biology, 2013, 36: 105-110.
[4] Delorenzi J C, Attias M, Gattass C R, et al. Antileishmanial activity of an indole alkaloid from Peschiera australis[J]. Antimicrobial Agents and chemotherapy, 2001, 45(5): 1349-1354.
[5] Arias H R, Tae H S, Micheli L, et al. Coronaridine congeners decrease neuropathic pain in mice and inhibit α9α10 nicotinic acetylcholine receptors and CaV2. 2 channels[J]. Neuropharmacology, 2020, 175: 108194.

Protocol

Cell experiment [1]:

Cell lines

Hep-2 cells

Preparation Method

Hep-2 cells (2×104 cells/mL) were incubated with 15.62, 31.25, 62.5, and 125µg/mL Coronaridine, respectively, for 6 hours at 37°C in a 5% CO2 atmosphere. Minor modifications were made to the alkaline comet assay. Three hundred cells were randomly selected from three replicate slides, for a total of=900 cells, and each Coronaridine concentration was analyzed. The cells were scored for damage and divided into five categories to calculate the DNA damage index. Doxorubicin 0.2μg/mL was used as a positive control, and 1% dimethyl sulfoxide was used as a solvent control.

Reaction Conditions

15.62, 31.25, 62.5, and 125µg/mL; 6h

Applications

Coronaridine treatment increased DNA damage in Hep-2 cell line in a concentration-dependent manner.
Animal experiment [2]:

Animal models

Male CD-1 albino mice

Preparation Method

Male CD-1 albino mice weighing approximately 22 to 25g at the beginning of the experiment were used in all experiments. Ten mice were housed in each cage (26 ×41cm). Animals were fed a standard laboratory diet and tap water AD libitum and maintained at 23±1°C on a 12-h day-night cycle with light at 7 am. Neuropathic pain was produced in mice (n=10/group) intermittently by intraperitoneal (i.p.) injection of 2.4mg/kg oxaliplatin (dissolved in 5% glucose solution) on days 1-3, 6-10, and 13-14. On day 15, a coronary contrast agent (Coronaridine) that induces antineuropathic pain was administered (p.o.) at the highest dose (72mg/kg), and a hole-plate test was performed 25 minutes later. The device consisted of a 40cm square plane with 16 flush cylindrical holes (3cm in diameter) distributed in a 4×4 equispaced grid. Each mouse was placed in the center of the plate and allowed to move freely for 5min at a time. Two light beams passed from the midpoint of the plane to the midpoint on opposite sides, thus dividing the plane into four equal quadrants, automatically signaling the movement of the animal across the plate surface for a total time of 5min (i.e., spontaneous activity). Miniature photocells were present in each of the 16 wells, and the mice were recorded for a total of 5min of hole exploration time. Higher or lower exploratory activity compared with control animals indicates anxiolytic or anxiogenic activity of the drug, respectively.

Dosage form

72mg/kg for 25min; p.o.

Applications

Coronaridine treatment decreased locomotor activity and reduced spontaneous mobility in mice.

References:
[1] Rizo W F, Ferreira L E, Colnaghi V, et al. Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A. DC in a human laryngeal epithelial carcinoma cell line (Hep-2)[J]. Genetics and molecular biology, 2013, 36: 105-110.
[2] Arias H R, Tae H S, Micheli L, et al. Coronaridine congeners decrease neuropathic pain in mice and inhibit α9α10 nicotinic acetylcholine receptors and CaV2. 2 channels[J]. Neuropharmacology, 2020, 175: 108194.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
467-77-6
Molecular Formula
C21H26N2O2
Molecular Weight
338.44
Storage
4°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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