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DAF-2 (Synonyms: 4,5-Diaminofluorescein)

Catalog No.GC43372

DAF-2 is a fluorescent detector for nitric oxide (NO) in cells and tissues.

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DAF-2 Chemical Structure

Cas No.: 205391-01-1

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100ug
$85.00
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250ug
$198.00
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500ug
$372.00
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1mg
$660.00
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Sample solution is provided at 25 µL, 10mM.

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Product Documents

Quality Control & SDS

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Background

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).[1],[2] It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.2 At neutral pH the detection limit for NO is 2-5 nM.

Reference:

[1]. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & Pharmaceutical Bulletin 46, 373-375 (1998).
[2]. Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Letters 427, 263-266 (1998).


Chemical Properties

Cas No. 205391-01-1 SDF
Synonyms 4,5-Diaminofluorescein
Canonical SMILES OC1=CC2=C(C=C1)C3(C(C=C(N)C(N)=C4)=C4C(O3)=O)C5=CC=C(O)C=C5O2
Formula C20H14N2O5 M.Wt 362.3
Solubility A solution in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.7601 mL 13.8007 mL 27.6014 mL
5 mM 0.552 mL 2.7601 mL 5.5203 mL
10 mM 0.276 mL 1.3801 mL 2.7601 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 33 reference(s) in Google Scholar.)

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