GLPBio

DB2313

Catalog No.: GC62222Purity:>98.00%
COASDSData Sheet
DB2313 is a potent transcription factor PU.1 inhibitor with an IC50 of 14nM.

Products are for research use only. Not for human use. We do not sell to patients.

DB2313
Cas No.: 2170606-74-1
1mgIn Stock
$42.00
1
5 mgIn Stock
$42.00
1
10 mgIn Stock
$147.00
1
25 mgIn Stock
$235.00
1
50 mgIn Stock
$349.00
1
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Description

DB2313 is a potent transcription factor PU.1 inhibitor with an IC50 of 14nM[1]. DB2313 disrupts the interaction of PU.1 with target gene promoters[2]. PU.1, a critical transcription factor in the hematopoietic system, plays key roles in myeloid and lymphoid cell differentiation, maintenance of hematopoietic stem cell functions, and the development of acute myeloid leukemia (AML)[3]. DB2313 is usually used in research related to AML and inflammation[4].

In vitro, DB2313 (10μg/ml; 72h) significantly inhibited the proliferation, migration, and invasion of GBM cells (U118MG and U87MG)[5].

In vivo, DB2313 (17mg/kg; every 2 days via intraperitoneal injection for 12 days) significantly suppressed tumor growth and enhanced the recruitment of CD4+ T helper 1 (Th1) and cytotoxic T/natural killer (NK) cells into tumors in the B16-OVA melanoma mouse model[6]. DB2313 (8mg/kg; intranasal administration; 5 days/week for 3 weeks) decreased airway mucus-secreting cell numbers and small airway collagen deposition, and significantly reduced airway hyperresponsiveness in a house dust mite (HDM)-induced experimental asthma mouse model[7].

References:
[1] Antony-Debre I, Paul A, Leite J, et al. Pharmacological inhibition of the transcription factor PU.1 in leukemia. J Clin Invest. 2017;127(12):4297-4313.
[2] Tu J, Chen W, Fang Y, et al. PU.1 promotes development of rheumatoid arthritis via repressing FLT3 in macrophages and fibroblast-like synoviocytes. Ann Rheum Dis. 2023;82(2):198-211.
[3] Fisher RC, Scott EW. Role of PU.1 in hematopoiesis. Stem Cells. 1998;16(1):25-37.
[4] Tu X, Kim RY, Brown AC, et al. Airway and parenchymal transcriptomics in a novel model of asthma and COPD overlap. J Allergy Clin Immunol. 2022;150(4):817-829.e6.
[5] Zhang S, Zhao S, Qi Y, et al. SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner. Mol Ther Nucleic Acids. 2022;27:699-717.
[6] Sleapnicov N, Ha SD, Zhong SJ, et al. Inhibition of the Transcription Factor PU.1 Suppresses Tumor Growth in Mice by Promoting the Recruitment of Cytotoxic Lymphocytes Through the CXCL9-CXCR3 Axis. Cancers (Basel). 2025;17(16):2684.
[7] Tu X, Gomez HM, Kim RY, et al. Airway and parenchyma transcriptomics in a house dust mite model of experimental asthma. Respir Res. 2023;24(1):32.

Protocol

Cell experiment [1]:

Cell lines

glioma cell lines U87MG and U118MG

Preparation Method

The glioma cell lines U87MG and U118MG were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin-streptomycin. All cells were maintained in a humidified incubator with 5% CO2 at 37℃. The cells were routinely tested for mycoplasma contamination. 2000 GBM cells were seeded in 96-well plates and measured cell viability using CCK-8 assay at 0, 24, 48, and 72h. GBM cells were treated with DMSO or DB2313 (10mg/mL). EdU assays were performed using an EdU assay kit with GBM cells seeded in well plates at 2×105 cells per well. 2×104 GBM cells were used for the Transwell assay. A total of 2×105 GBM cells were cultured in spheroid formation ECM for 72h to generate tumor spheroids and used for the 3D tumor spheroid invasion assay with a 96-well 3D spheroid BME cell invasion assay kit. Images were taken at 0, 24, 48, and 72h using microscope.

Reaction Conditions

10μg/ml; 72h

Applications

DB2313 significantly inhibited the proliferation, migration, and invasion of GBM cells (U118MG and U87MG).
Animal experiment [2]:

Animal models

Female wild-type BALB/c mice

Preparation Method

Female wild-type BALB/c mice were housed under specific pathogen-free conditions and maintained on a 12-hour day-night cycle with water and regular chow available ad libitum. Mice were treated intranasally with house dust mite (HDM; Dermatophagoides pteronyssinus) extract (25µg in 30µL PBS or saline (vehicle), 5 days per week for 3 weeks. One group of HDM-treated mice were treated with DB2313 (8mg/kg in PBS). Bronchoalveolar lavage fluid was collected. Mucus-secreting cells (MSCs) were stained and counted. Collagen was stained and analyzed. Airway hyperresponsiveness was assessed using the forced oscillation technique.

Dosage form

8mg/kg; intranasal administration; 5 days/week for 3 weeks

Applications

DB2313 decreased airway mucus-secreting cell numbers and small airway collagen deposition, and significantly reduced airway hyperresponsiveness in a house dust mite (HDM)-induced experimental asthma mouse model.

References:
[1] Zhang S, Zhao S, Qi Y, et al. SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner. Mol Ther Nucleic Acids. 2022;27:699-717.
[2] Tu X, Gomez HM, Kim RY, et al. Airway and parenchyma transcriptomics in a house dust mite model of experimental asthma. Respir Res. 2023;24(1):32.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
2170606-74-1
Molecular Formula
C42H41FN8O2
Molecular Weight
708.83
Solubility
DMSO : 3.7 mg/mL (5.22 mM; ultrasonic and warming and heat to 70°C)
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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