GLPBio

Budesonide

Catalog No.: GC11897Purity:>99.50%
COASDSData Sheet
Budesonid, ein inhalatives Glucocortic-Steroid, ist ein oral wirksamer Glucocorticoid-Rezeptor-Agonist.

Die Produkte sind ausschließlich für Forschungszwecke bestimmt. Nicht zur Anwendung am Menschen. Wir verkaufen nicht an Patienten.

Budesonide
Cas No.: 51333-22-3
10mM (in 1mL DMSO)In Stock
$41.00
1
100mgIn Stock
$37.00
1
200mgIn Stock
$69.00
1
500mgIn Stock
$156.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Zitate (Citations)

Für dieses Produkt sind derzeit keine Zitate verfügbar. Nachfolgend finden Sie ausgewählte Publikationen, die GlpBio-Produkte zitieren.
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

Beschreibung (Description)

Budesonide is a glucocorticoid with potent local anti-inflammatory effects. Budesonide enhances the stability of endothelial cells, smooth muscle cells, and lysosomal membranes, inhibits immune responses, and reduces antibody synthesis, thereby decreasing the release and activity of allergic mediators such as histamine. Budesonide is used in the treatment of bronchial asthma, asthmatic chronic bronchitis, allergic rhinitis, nasal polyps, Crohn's disease, and ulcerative colitis[1-4].

In vitro, Budesonide (1μM–25μM) was used to treat LS180 and Caco-2 intestinal cell lines for 48h–72h. Budesonide induced the expression of MDR1/P-gp in LS180 cells and down-regulated the expression of MDR1/P-gp in Caco-2 cells[5]. Budesonide (0.1–25μM) was co-treated with SARS-CoV-2 (MOI=0.1) in Vero E6 cells for 24 hours. Budesonide significantly reduced the viral titer in Vero E6 cells without affecting cell viability[6].

In vivo, Budesonide (10mg/kg/day) was administered daily via oral gavage to female C57BL/6 mice for 5 days. Budesonide significantly increased the mortality rate in Clostridium difficile-infected mice[7]. Budesonide (1mg) combined with Calcitriol (100ng) was administered daily via nebulized inhalation and intraperitoneal injection to asthmatic model BALB/c mice for 8 weeks. Budesonide and Calcitriol synergistically inhibited airway remodeling in asthmatic mice[8].

References:
[1] Olivas I, Cobreros M, Londoño MC, et al. Budesonide in the first line treatment of patients with autoimmune hepatitis. Gastroenterol Hepatol. 2022 Aug-Sep;45(7):561-570.
[2] Grossmann C, Scholz T, Rochel M, et al. Transactivation via the human glucocorticoid and mineralocorticoid receptor by therapeutically used steroids in CV-1 cells: a comparison of their glucocorticoid and mineralocorticoid properties. Eur J Endocrinol. 2004 Sep;151(3):397-406.
[3] Dong L, Zhu YH, Liu DX, et al. Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice. J Immunol Res. 2019 Feb 27;2019:7264383.
[4] Pereira MA, Tao L, Liu Y, et al. Modulation by budesonide of DNA methylation and mRNA expression in mouse lung tumors. Int J Cancer. 2007 Mar 1;120(5):1150-3.
[5] Maier A, Zimmermann C, Beglinger C, et al. Effects of budesonide on P-glycoprotein expression in intestinal cell lines. Br J Pharmacol. 2007 Feb;150(3):361-8.
[6] Heinen N, Meister TL, Klöhn M, et al. Antiviral Effect of Budesonide against SARS-CoV-2. Viruses. 2021 Jul 20;13(7):1411.
[7] Lin Q, Li Z, Lu L, et al. Budesonide, an anti-inflammatory drug, exacerbate clostridioides difficile colitis in mice. Biomed Pharmacother. 2023 Nov;167:115489.
[8] Qian J, Xu Y, Yu Z. Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice. Can Respir J. 2018 May 2;2018:5259240.

Protokoll (Protocol)

Cell experiment [1]:

Cell lines

LS180 and Caco-2 cells (human intestinal cell lines)

Preparation Method

LS180 and Caco-2 cells were cultured in Dulbecco's modified Eagle's medium with Glutamax-I, supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 1% sodium pyruvate, 50μg/ml gentamycin. Cells were seeded into 12-well plastic culture dishes and maintained in a humidified 37°C incubator with 5% CO2. Cells were treated with Budesonide (1μM–25μM).

Reaction Conditions

1μM–25μM; 48h

Applications

Budesonide induced MDR1/P-glycoprotein expression in LS180 cells and down-regulated MDR1/P-glycoprotein expression in Caco-2 cells. These changes were confirmed at mRNA, protein, and functional levels.

Animal experiment [2]:

Animal models

Female BALB/c mice (6-8 weeks old)

Preparation Method

Mice were sensitized by intraperitoneal injection of 50μg ovalbumin (Ova) with 1mg aluminum hydroxide on days 1 and 14. From day 21, mice were challenged by inhalation of 1% Ova solution for 30min once daily for eight weeks to establish an asthma model. In the Budesonide treatment group, 0.5h before each Ova challenge, mice inhaled 5mL PBS containing 1mg Budesonide via a nebulizer over 30min.

Dosage form

1mg; inhalation; daily administration before each Ova challenge; 8 weeks

Applications

Budesonide monotherapy alleviated pathological changes including airway eosinophil infiltration, mucus secretion, and collagen proliferation in asthmatic mice. Budesonide inhibited the high expression of collagen type I (COL I), collagen type III (COL III), and α-smooth muscle actin (α-SMA), downregulated the expression of TGFβ type I receptor (TGFβRI), phosphorylated Smad2 (pSmad2), and phosphorylated Smad3 (pSmad3), and upregulated the expression of Smad7 in lung tissue. Budesonide decreased the expression of microRNA-21 (miR-21) in lung tissue.

References:
[1] Maier A, Zimmermann C, Beglinger C, et al. Effects of budesonide on P-glycoprotein expression in intestinal cell lines. Br J Pharmacol. 2007 Feb;150(3):361-8.
[2] Qian J, Xu Y, Yu Z. Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice. Can Respir J. 2018 May 2;2018:5259240.

Produktdokumente (Product Documents)

Purity:>99.50%Appearance:A solid
COASDSData Sheet

Chemische Eigenschaften (Chemical Properties)

CAS-Nr.
51333-22-3
Chemischer Name
(6aR,6bS,7S,8aS,8bS,11aR,12aS,12bS)-7-hydroxy-8b-(2-hydroxyacetyl)-6a,8a-dimethyl-10-propyl-6a,6b,7,8,8a,8b,11a,12,12a,12b-decahydro-1H-naphtho[2',1':4,5]indeno[1,2-d][1,3]dioxol-4(2H)-one
SMILES
O=C(C([H])([H])O[H])[C@@]12OC(C([H])([H])C([H])([H])C([H])([H])[H])([H])O[C@]1([H])C([H])([H])[C@]([C@@](C([H])([H])C3([H])[H])([H])[C@@]4([H])[C@@](C([H])([H])[H])(C([H])=C5[H])C3=C([H])C5=O)([H])[C@]2(C([H])([H])[H])C([H])([H])[C@]4([H])O[H]
Summenformel
C25H34O6
Molekulargewicht
430.53
Löslichkeit
≥ 20.2mg/mL in DMSO
Lagerung
Store at -20°C
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.

Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit

g/mol

Reviews

Review for Budesonide

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%