eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) (387-394) [Multiple species] (Synonyms: H2N-Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu-OH ) |
| Katalog-Nr.GP10018 |
Elongation factor-1 subunit
Products are for research use only. Not for human use. We do not sell to patients.
![eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) (387-394) [Multiple species] Chemische Struktur](/media/struct/GP1/GP10018.png "eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) (387-394) [Multiple species] Chemische Struktur")
Sample solution is provided at 25 µL, 10mM.
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Sequence: LEU-GLU-ASP-GLY-PRO-LYS-PHE-LEU
eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes1.
There are two forms of eEF1A in eukaryotes, eEF1A1 and eEF1A2, both of which possess the same role in protein synthesis2. eEF1A2 was reported to be exclusively expressed in brain, heart, and skeletal muscle, whereas eEF1A1 is well known to be ubiquitously expressed3.
tetraploid cells produced by impaired chromosomal condensation are eliminated by a novel type of cell death different from caspase-dependent apoptosis. The cell death was associated with downregulation of eukaryotic translation elongation factor-1 a 1 (eEF1A1/EF-1a) expression in conjunction with accumulation of its mRNA in processing bodies (P bodies). Importantly, expression of exogenous eEF1A1 was shown to inhibit the caspase-independent cell death, and a similar cell death was observed after inducing the expression of short hairpin RNA speci?c for eEF1A12.
References:
1. "Entrez Gene: EEF1A1 eukaryotic translation elongation factor 1 alpha 1"
2. Kahns S, LundA, Kristensen P, Knudsen CR, ClarkBF, Cavallius J et al. The elongationfactor 1 A-2 isoform from rabbit: cloning of the cDNA and characterization of the protein. Nucleic Acids Res 1998; 26: 1884-1890
3. Lee S, Francoeur AM, Liu S, Wang E. Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family. J Biol Chem 1992; 267: 24064-24068.
4. Y Kobayashi and S Yonehara. Cell Death and Differentiation (2009) 16, 139-150
| Cas No. | SDF | ||
| Überlieferungen | H2N-Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu-OH | ||
| Canonical SMILES | NC(CC(C)C)C(NC(CCC(O)=O)C(NC(CC(O)=O)C(NCC(N(CCC1)C1C(NC(CCCCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O | ||
| Formula | C43H67N9O13 | M.Wt | 918.04 |
| Löslichkeit | ≥91.8mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0893 mL | 5.4464 mL | 10.8928 mL |
| 5 mM | 0.2179 mL | 1.0893 mL | 2.1786 mL |
| 10 mM | 0.1089 mL | 0.5446 mL | 1.0893 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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