Flipper-TR probe (Compound FliptR probe) is a fluorescent probe specifically designed to measure cell membrane tension. Flipper-TR probe reports changes in membrane tension through variations in its fluorescence lifetime. Flipper-TR probe is applicable to a wide range of organisms including bacteria, yeast, mammals, and plants.
Usage Guidelines: Storage: Flipper-TR should be stored at -20°C upon receipt.Probe Preparation: Dissolve the probe in androus DMSO to create a 1 mM stock solution, which should be stored at -20 °C or lower.Staining Solution Preparation: Prior to use, dilute Flipper-TR to the desired concentration (typically starting at 1 µM) in cell culture medium.Staining Protocol: After cells reach the desired density, replace the culture medium with the staining solution to cover all cells, then incubate at 37°C in a humidified atmosphere with 5 % CO2 for 15 minutes before imaging.
References:
[1]. Colom A, et al. A fluorescent membrane tension probe. Nat Chem. 2018 Nov;10(11):1118-1125.