HOE-S 785026 (Synonyms: meta-Hoechst) |
Katalog-Nr.GC36248 |
HOE-S 785026 ist ein blau fluoreszierender Farbstoff, der als Zellfarbstoff fÜr DNA verwendet werden kann.
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Cas No.: 132869-83-1
Sample solution is provided at 25 µL, 10mM.
HOE-S 785026 is a Hoechst series dye that is commonly used for nuclear labeling staining of living cells. Hoechst dye can be used to monitor cell viability by tracking changes in its emission spectrum0. As slightly groove-binding DNA stains with AT selectivity, Hoechst dyes are able to bind to all nucleic acids, but they show greater fluorescence enhancement for AT-rich double-stranded DNA strands compared to GC-rich strands [ 1]. This property has been used to identify Q bands in chromosomes, which are AT base pair-rich regions that fluoresce brightly when stained with quinacrine dye [2]. The fluorescence intensity of Hoechst dye increases as the pH of the solution increases.
References:
[1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′, 6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression . 1988 Feb 28;949(2):158-68.
[2]. Weisblum B, Haenssler E. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA. Chromosoma. 1974 Sep;46(3):255-60.
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