GLPBio

JY-2

Catalog No.: GD96632Purity:>99.75%
COASDSData Sheet
JY-2 is a moderately selective FoxO1 inhibitor with IC50 of 22μM, JY-2 has moderate inhibitory effect on FoxO3a and FoxO4 too.

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JY-2
Cas No.: 339103-05-8
5mgIn Stock
$54.00
1
10mgIn Stock
$90.00
1
25mgIn Stock
$180.00
1
50mgIn Stock
$270.00
1
100mgIn Stock
$405.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Zitate (Citations)

2 publicationsPublikationen von Forschern, die dieses Produkt verwenden

Beschreibung (Description)

JY-2 is a moderately selective FoxO1 inhibitor with IC50 of 22μM, JY-2 has moderate inhibitory effect on FoxO3a and FoxO4 too [1].

JY-2(0.5mM; 24h) reduced the palmitic acid (PA)-stimulated mRNA expression of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), two key enzymes involved in gluconeogenesis in HepG2 cells[1]. FoxO3 inhibitor JY-2 can antagonize cell iron death induced by Genistein via FoxO3/SLC7A11/GPX4 signaling pathway[2].

JY-2(50, 100, 200mg/kg; p.o; 3 times) improved glucose tolerance, in parallel with a reduced mRNA expression of gluconeogenic genes in high fat-diet induced obese and diabetic (DIO) mice models[1].

References:
[1]. Choi HE, Kim Y, et,al. Novel FoxO1 inhibitor, JY-2, ameliorates palmitic acid-induced lipotoxicity and gluconeogenesis in a murine model. Eur J Pharmacol. 2021 May 15;899:174011. doi: 10.1016/j.ejphar.2021.174011. Epub 2021 Mar 9. PMID: 33705803.
[2]. Liu L, Qiu Y, et,al. Genistein Induces Ferroptosis in Colorectal Cancer Cells via FoxO3/SLC7A11/GPX4 Signaling Pathway. J Cancer. 2024 Nov 4;15(20):6741-6753. doi: 10.7150/jca.95775. PMID: 39668836; PMCID: PMC11632993.

Protokoll (Protocol)

Cell experiment [1]:

Cell lines

HepG2 cells

Preparation Method

Cells were cultured in DMEM supplemented with 1% penicillin/streptomycin and 10% FBS, and maintained at 37 ℃ in a humidified atmosphere of 5% CO2. Cells were plated in cell culture flasks for at least 24h before treatment. Batches of cells were seeded in 24-well plates at a density of 2 × 105 cells/well and incubated with various concentrations (10, 50, or 100μM) of JY-2 for 1h, followed by PA (0.5mM) treatment for the indicated time.

Reaction Conditions

0.5mM; 24h

Applications

JY-2 reduces PA-induced lipotoxicity in HepG2 cells.
Animal experiment [1]:

Animal models

C57BL/6J mice

Preparation Method

JY-2 (50, 100, 200mg/kg) was orally administered to mice three times for two days (9:00 AM, 7:00 PM, 9:00 AM on the next day), and pyruvate tolerance challenge was carried out after overnight fasting.

Dosage form

50, 100, 200mg/kg; p.o; 3 times

Applications

JY-2 improves glucose intolerance in vivo.

References:
[1]. Choi HE, Kim Y, et,al. Novel FoxO1 inhibitor, JY-2, ameliorates palmitic acid-induced lipotoxicity and gluconeogenesis in a murine model. Eur J Pharmacol. 2021 May 15;899:174011. doi: 10.1016/j.ejphar.2021.174011. Epub 2021 Mar 9. PMID: 33705803.

Produktdokumente (Product Documents)

Purity:>99.75%Appearance:A solid
COASDSData Sheet

Chemische Eigenschaften (Chemical Properties)

CAS-Nr.
339103-05-8
Summenformel
C13H7Cl2N3O
Molekulargewicht
292.12
Löslichkeit
80mg/mL in DMSO (ultrasonic and warming and heat to 60°C)
Lagerung
-20°C
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.

Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit

g/mol

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