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pyCTZ

Katalog-Nr.GC70727 Copy One-Click Copy Product Info

pyCTZ (Pyridyl CTZ), ein Pyridyl Coelenterazin (CTZ) Analog, und ist ein ATP-unabhängiges Pyridyl Substrat der LumiLuc Luciferase.

Products are for research use only. Not for human use. We do not sell to patients.

pyCTZ Chemische Struktur

Cas No.: 2125486-34-0

Größe Preis Lagerbestand Menge
1 mg
117,00 $
Auf Lager
5 mg
270,00 $
Auf Lager
10 mg
468,00 $
Auf Lager
25 mg
882,00 $
Auf Lager

Tel:(909) 407-4943 Email: sales@glpbio.com


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Sample solution is provided at 25 µL, 10mM.



Description of pyCTZ

pyCTZ (Pyridyl CTZ), a pyridyl Coelenterazine (CTZ) analog, and is an ATP-independent pyridyl substrate of LumiLuc luciferase. pyCTZ generates strong blue bioluminescence in the presence of luciferases. pyCTZ can be used for aequorin-based calcium sensing.

pyCTZ is comparable to native CTZ for bioluminescence intensity when pairs with aequorin[1].

References:
[1]. Yeh HW, et, al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.

Chemical Properties of pyCTZ

Cas No. 2125486-34-0 SDF
Formula C25H20N4O M.Wt 392.45
Löslichkeit Methanol : 41.67 mg/mL (106.18 mM; Need ultrasonic)DMSO : 33.33 mg/mL (84.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Storage -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of pyCTZ

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.5481 mL 12.7405 mL 25.481 mL
5 mM 509.6 μL 2.5481 mL 5.0962 mL
10 mM 254.8 μL 1.274 mL 2.5481 mL
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In vivo Formulation Calculator (Clear solution) of pyCTZ

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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