Thiazole Orange
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| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $54.00 | In Stock | 1 | |
| 100mg | $49.00 | In Stock | 1 | |
| 250mg | $98.00 | In Stock | 1 |
Zitate (Citations)
1 publicationsPublikationen von Forschern, die dieses Produkt verwendenBeschreibung (Description)
Thiazole Orange is an asymmetrical cyanine dye belonging to the nucleic acid fluorescent dye family. Thiazole Orange exhibits a significant fluorescence enhancement effect upon binding to nucleic acids, particularly RNA, a property that makes Thiazole Orange widely applicable in biomolecular detection[1]. Thiazole Orange is capable of penetrating the membrane of living cells and demonstrates a high quantum yield upon nucleic acid binding. Thiazole Orange maximum excitation wavelength (λex) is approximately 510nm (with strong excitation also at 488nm and 470nm), and Thiazole Orange maximum emission wavelength (λem) is approximately 527nm[2].
References:
[1] O'Neil CS, Beach JL, Gruber TD. Thiazole orange as an everyday replacement for ethidium bromide and costly DNA dyes for electrophoresis. Electrophoresis. 2018 Jun;39(12):1474-1477.
[2] Lee LG, Chen CH, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry. 1986 Nov;7(6):508-17.
Protokoll (Protocol)
Reticulocyte Counting Using Thiazole Orange
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
(1) Reagent Preparation: Dissolve Thiazole Orange in methanol to prepare a stock solution with a concentration of 1mg/mL. Dilute the Thiazole Orange stock solution with phosphate-buffered saline (PBS) containing 2mM EDTA and 0.02% sodium azide. The recommended working concentration is 0.3μM. The diluted dye solution can be stored stably for at least one week at room temperature protected from light.
(2) Sample Preparation: Use whole blood samples anticoagulated with EDTA. Take 5μL of the whole blood sample and add to 1mL of the diluted Thiazole Orange working solution. Incubate at room temperature, protected from light, for 1 hour.
(3) Flow Cytometry Analysis: Analyze using a flow cytometer equipped with a 488nm argon-ion laser. Set the standard FITC filter configuration (e.g., 530/30nm bandpass filter) to detect green fluorescence. Set the analysis rate to 500-1000 cells per second, and collect at least 10,000 cells per sample. The flow cytometry software automatically calculates the percentage of cells with fluorescence intensity above the set threshold, which corresponds to the reticulocyte percentage.
Precautions:
(1) The incubation time can range from 30 to 90 minutes with minimal impact on result accuracy; choosing 1 hour is most convenient.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Lee LG, Chen CH, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry. 1986 Nov;7(6):508-17.
Produktdokumente (Product Documents)
Chemische Eigenschaften (Chemical Properties)
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit
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