Tracer AL244 is a BODIPY-based fluorescent tracer and also a TRIM21 binder. Tracer AL244 enables evaluation of TRIM21 target binding in both intact and permeabilized cells [1].
In Vitro, Tracer AL244 (6 nM-3 µM; 2 h) generates strong BRET signals in HEK293T cells expressing full-length human TRIM21 with a C-terminally fused NanoLuc, enabling live-cell target binding assays[1]. Tracer AL244 generates strong BRET signals and a favorable assay window when interacting with C-terminally fused NanoLuc-TRIM21, whereas it produces weak signals when interacting with N-terminally fused NanoLuc-TRIM21[1].
References:
[1]. Kim Y, et al. Crystallographic fragment screening reveals ligand hotspots in TRIM21 PRY-SPRY domain. Communications chemistry. 2025 Jun 13;8(1):185.
In Vitro, Tracer AL244 (6 nM-3 µM; 2 h) generates strong BRET signals in HEK293T cells expressing full-length human TRIM21 with a C-terminally fused NanoLuc, enabling live-cell target binding assays[1]. Tracer AL244 generates strong BRET signals and a favorable assay window when interacting with C-terminally fused NanoLuc-TRIM21, whereas it produces weak signals when interacting with N-terminally fused NanoLuc-TRIM21[1].
References:
[1]. Kim Y, et al. Crystallographic fragment screening reveals ligand hotspots in TRIM21 PRY-SPRY domain. Communications chemistry. 2025 Jun 13;8(1):185.