GLPBio

Trehalose (hydrate)

Catalog No.: GC45075Purity:>98.00%Synonyms: D-(+)-Trehalose
COASDSData Sheet
Trehalose (hydrate) is a natural, safe, non-specific disaccharide osmoprotectant.

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Trehalose (hydrate)
Cas No.: 6138-23-4
100mgIn Stock
$6.00
1
500mgIn Stock
$10.00
1
1gIn Stock
$14.00
1
5gIn Stock
$28.00
1
10gIn Stock
$39.00
1
25gIn Stock
$58.00
1
50gIn Stock
$69.00
1
100gIn Stock
$83.00
1
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Zitate (Citations)

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    641, 529–536 (2025)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    388(6745) (2025)
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    387(6734) (2025)
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    35, 97–116 (2025)
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    33, 546–561 (2023)
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    33, 904–922 (2023)
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    Cell Research
    31, 1291–1307 (2021)

Beschreibung (Description)

Trehalose (hydrate) is a natural, safe, non-specific disaccharide osmoprotectant[1]. Trehalose (hydrate) exerts anti-desiccation, anti-freezing, and anti-oxidative effects by stabilizing protein and membrane structures and maintaining osmotic pressure[2]. Trehalose (hydrate) is widely used in studies of metabolic diseases, neurodegenerative disorders, and food–drug delivery systems[3][4].

In vitro, Trehalose (hydrate) (100mM; 72h) significantly increases the viability of H9c2 cardiomyocytes exposed to high glucose (33mM), decreases the Bax/Bcl-2 ratio and cleaved-caspase-3 protein levels, suppresses NALP3/caspase-1/IL-1β/IL-18-mediated pyroptosis, restores the expression of Beclin-1 and Atg5 proteins as well as the LC3-II/I ratio, and reverses high-glucose–induced autophagic impairment[5].

In vivo, Trehalose (hydrate) (4% w/v in drinking water; 14 days; ad libitum) fully reversed the memory deficit induced by bilateral i.c.v. Aβ25–35 injection, restoring step-through latency in the passive avoidance test to control levels in APP/PS1 mice, while significantly reducing hippocampal CA1 Aβ fluorescence intensity and IBA1-positive microglial density, and restoring neuronal density to control values as shown by Nissl staining[6]. Trehalose (hydrate) (2% w/v in drinking water; ad libitum from 64 days of age until death) significantly delayed disease onset and extended mice lifespan, doubled motor neuron survival in the L4–5 spinal cord, and reduced spinal insoluble SOD1 aggregates in SOD1G93A mice[7].

References:
[1] Megarry AJ, Booth J, Burley J. Amorphous trehalose dihydrate by cryogenic milling. Carbohydr Res. 2011;346(8):1061-1064.
[2] Chen A, Tapia H, Goddard JM, Gibney PA. Trehalose and its applications in the food industry. Compr Rev Food Sci Food Saf. 2022;21(6):5004-5037.
[3] Sharma E, Shruti PS, Singh S, et al. Trehalose and its Diverse Biological Potential. Curr Protein Pept Sci. 2023;24(6):503-517.
[4] Sevriev B, Dimitrova S, Kehayova G, Dragomanova S. Trehalose: Neuroprotective Effects and Mechanisms-An Updated Review. NeuroSci. 2024;5(4):429-444.
[5] Liu Y, Wu S, Zhao Q, et al. Trehalose Ameliorates Diabetic Cardiomyopathy: Role of the PK2/PKR Pathway. Oxid Med Cell Longev. 2021;2021:6779559.
[6] Pupyshev AB, Akopyan AA, Tenditnik MV, et al. Alimentary Treatment with Trehalose in a Pharmacological Model of Alzheimer's Disease in Mice: Effects of Different Dosages and Treatment Regimens. Pharmaceutics. 2024;16(6):813.
[7] Zhang X, Chen S, Song L, et al. MTOR-independent, autophagic enhancer trehalose prolongs motor neuron survival and ameliorates the autophagic flux defect in a mouse model of amyotrophic lateral sclerosis. Autophagy. 2014;10(4):588-602.

Protokoll (Protocol)

Cell experiment [1]:

Cell lines

Rat H9c2 cardiomyocytes

Preparation Method

Rat H9c2 cardiomyocytes were cultured in DMEM low-sugar medium supplemented with 10% FBS and a 1% penicillin-streptomycin solution at 37°C and 5% CO2 in a humidified environment. Cells were incubated with 33mM high glucose for 72h with or without different concentrations of Trehalose (hydrate) (100mM). The MTT assay was performed according to the manufacturer’s instructions.

Reaction Conditions

100mM; 72h

Applications

Trehalose (hydrate) significantly increases the viability of H9c2 cardiomyocytes exposed to high glucose.

Animal experiment [2]:

Animal models

Male SOD1G93A mice

Preparation Method

Male SOD1G93A mice were randomly divided into 3 groups (Tg-NT, Tg-Suc, Tg-Tre) with 21 mice in each group and these mice were housed under the conditions of constant temperature and controlled lighting (light/dark period 12/12h). For Tg-Tre mice, 2% Trehalose (hydrate) containing water was given to SOD1G93A mice through ad libitum consumption, starting at 64d of age and continuing until the day the mice died. For Tg-Suc mice, 2% sucrose-containing water was given to mice as a vehicle control. For Tg-NT mice, no treatment was given to SOD1G93A mice. In addition, 15 of the age-matched WT littermates were used as controls. Rotarod performance was measured in SOD1G93A mice starting at 80d of age. SOD1G93A mice developed complete paralysis roughly at around 126d of age. For life-span analysis, the date of “death” (or terminal impairment) was defined as the day when the mice could not right itself within 30s after being placed on its back.

Dosage form

2% w/v in drinking water; ad libitum from 64 days of age until death

Applications

Trehalose (hydrate) significantly delayed disease onset and extended mice lifespan in SOD1G93A mice.

References:
[1] Liu Y, Wu S, Zhao Q, et al. Trehalose Ameliorates Diabetic Cardiomyopathy: Role of the PK2/PKR Pathway. Oxid Med Cell Longev. 2021;2021:6779559.
[2] Zhang X, Chen S, Song L, et al. MTOR-independent, autophagic enhancer trehalose prolongs motor neuron survival and ameliorates the autophagic flux defect in a mouse model of amyotrophic lateral sclerosis. Autophagy. 2014;10(4):588-602.

Produktdokumente (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemische Eigenschaften (Chemical Properties)

CAS-Nr.
6138-23-4
Synonyme
D-(+)-Trehalose
SMILES
OC[C@@H](O1)[C@@H](O)[C@H](O)[C@@H](O)[C@@]1([H])O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O2.O.O
Summenformel
C12H22O11•2H2O
Molekulargewicht
378.3
Löslichkeit
Water : 150 mg/mL (396.48 mM)
Lagerung
Store at RT
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.

Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit

g/mol

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