DMOG (Synonyms: Dimethyloxallyl Glycine) |
Catalog No.GC16973 |
An HIF-PH inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 89464-63-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
B1 cells |
Preparation Method |
B1 cells were FACS sorted from total peritoneal cells. Cells were cultured in the presence of DMOG (1 mM) for 2 h followed by stimulation. |
Reaction Conditions |
1 mM DMOG for 2 hours |
Applications |
The hydroxylase inhibitor DMOG reduce susceptibility to endotoxemia by tolerating cells to LPS activation and promoting M2 polarization and subsequent up-regulation of IL-10 by peritoneal B1 cells. |
Animal experiment [2]: | |
Animal models |
BALB/c and C57BL/6 mice |
Preparation Method |
Intraperitoneal injection of DMOG |
Dosage form |
8 mg DMOG / mouse |
Applications |
DMOG significantly increased survival after LPS-induced shock. DMOG up-regulated the expression of IL-10 in peritoneal B-1 cells. Mice treated with DMOG before surgery developed severe worsening of disease symptoms and significantly increased mortality. |
References: [1]. Hams E, Saunders SP, et,al. The hydroxylase inhibitor dimethyloxallyl glycine attenuates endotoxic shock via alternative activation of macrophages and IL-10 production by B1 cells. Shock. 2011 Sep;36(3):295-302. doi: 10.1097/SHK.0b013e318225ad7e. PMID: 21844787; PMCID: PMC3157050. |
DMOG(dihydroxyalanine) is an antagonist of α-ketoglutarate cofactor and an inhibitor of HIF-proline hydroxylase, leading to the stability and accumulation of HIF-1α protein in the nucleus[4].
DMOG reduce susceptibility to endotoxemia by tolerating cells to LPS activation and promoting M2 polarization and subsequent up-regulation of IL-10 by peritoneal B1 cells[1]. DMOG inhibited hydroxyproline synthesis from chick embryo lung, with IC50 values of 9.3 μM and 3.7 μM corresponding to tissue and medium sources, respectively[2].The combination of DMOG and nSi exerted admirable effects on periodontal tissue regeneration. DMOG/nSi-PLGA fibrous membranes could enhance and orchestrate osteogenesis-angiogenesis[4].Inhibition of hydroxylase by oxygen sensing leads to upregulation of the transcription factors HIF-1α and NF-β under normal oxygen in vitro,0.1 to 1mM DMOG can stabilize the expression of HIF-1α[3].DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[8]. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[5][6]. DMOG induces cell autophagy and protect cells from a subsequent OGD insult.[7].
DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[5]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[6].
References:
[1]: Hams E, Saunders SP, et,al. The hydroxylase inhibitor dimethyloxallyl glycine attenuates endotoxic shock via alternative activation of macrophages and IL-10 production by B1 cells. Shock. 2011 Sep;36(3):295-302. doi: 10.1097/SHK.0b013e318225ad7e. PMID: 21844787; PMCID: PMC3157050.
[2]: Baader E, Tschank G, et,al. Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues. Biochem J. 1994 Jun 1;300 ( Pt 2)(Pt 2):525-30. doi: 10.1042/bj3000525. PMID: 8002959; PMCID: PMC1138193.
[3]: Jaakkola P, Mole DR, et,al. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science. 2001 Apr 20;292(5516):468-72. doi: 10.1126/science.1059796. Epub 2001 Apr 5. PMID: 11292861.
[4]: Shang L, Liu Z, Ma B, Shao J, Wang B, Ma C, Ge S. Dimethyloxallyl glycine/nanosilicates-loaded osteogenic/angiogenic difunctional fibrous structure for functional periodontal tissue regeneration. Bioact Mater. 2020 Oct 26;6(4):1175-1188. doi: 10.1016/j.bioactmat.2020.10.010. PMID: 33163699; PMCID: PMC7593348.
[5]: Milkiewicz M, Pugh CW, et,al. Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice. J Physiol. 2004 Oct 1;560(Pt 1):21-6. doi: 10.1113/jphysiol.2004.069757. Epub 2004 Aug 19. PMID: 15319416; PMCID: PMC1665195.
[6]: Li X, Zhao H, et,al. Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8. doi: 10.1093/abbs/gmt132. Epub 2014 Jan 3. PMID: 24389644.
[7]: Singh A, Wilson JW, et,al. Hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors induce autophagy and have a protective effect in an in-vitro ischaemia model. Sci Rep. 2020 Jan 31;10(1):1597. doi: 10.1038/s41598-020-58482-w. Erratum in: Sci Rep. 2020 Apr 8;10(1):6041. PMID: 32005890; PMCID: PMC6994562.
[8]: Schultz K, Murthy V, et,al. Prolyl hydroxylase 2 deficiency limits proliferation of vascular smooth muscle cells by hypoxia-inducible factor-1{alpha}-dependent mechanisms. Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L921-7. doi: 10.1152/ajplung.90393.2008. Epub 2009 Mar 20. PMID: 19304911; PMCID: PMC2692800.
Cas No. | 89464-63-1 | SDF | |
Synonyms | Dimethyloxallyl Glycine | ||
Chemical Name | methyl 2-[(2-methoxy-2-oxoethyl)amino]-2-oxoacetate | ||
Canonical SMILES | COC(=O)CNC(=O)C(=O)OC | ||
Formula | C6H9NO5 | M.Wt | 175.14 |
Solubility | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 5.7097 mL | 28.5486 mL | 57.0972 mL |
5 mM | 1.1419 mL | 5.7097 mL | 11.4194 mL |
10 mM | 0.571 mL | 2.8549 mL | 5.7097 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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