FeTPPS |
Catalog No.GC43663
One-Click Copy Product Info
|
FeTPPS is a metalloporphyrin-based ONOO⁻ decomposition catalyst.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 90384-82-0
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
FeTPPS is a metalloporphyrin-based ONOO⁻ decomposition catalyst [1]. FeTPPS catalyzes the decomposition of peroxynitrite (ONOO⁻), reducing its nitrating/oxidative damage to proteins, DNA, and lipids [2-3]. FeTPPS is primarily used in the study of oxidative stress-related disease models [4].
In cardiomyocytes, FeTPPS (50μM; 24h) reduces cell death caused by SIN-1 and SIN-1 plus H2O2 [5]. In spermatids, exposure to FeTPPS (25-50μM; 24h) ameliorates nitrosative stress [6].
In controlled cortical impact injury (CCI) mice model, FeTPPS (10mg/kg, 30mg/kg; ip; two injections) reduces secondary damage and improves neurobehavioral functions after traumatic brain injury. [7]. In a high-fat diet mice model, FeTPPS (50-60mg/kg; po; 8 weeks) treatment more than doubled the insulin-induced drop in blood glucose levels [8].
References:
[1]. Haber A, Gross Z. Catalytic antioxidant therapy by metallodrugs: lessons from metallocorroles[J]. Chemical Communications, 2015, 51(27): 5812-5827.
[2]. Uribe P, Barra J, Painen K, et al. FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa[J]. Antioxidants, 2023, 12(6): 1272.
[3]. Zhang P, Ma L, Yang Z, et al. 5, 10, 15, 20-Tetrakis (4-sulfonatophenyl) porphyrinato iron (III) chloride (FeTPPS), a peroxynitrite decomposition catalyst, catalyzes protein tyrosine nitration in the presence of hydrogen peroxide and nitrite[J]. Journal of Inorganic Biochemistry, 2018, 183: 9-17.
[4]. Duan X, Wen Z, Shen H, et al. Intracerebral hemorrhage, oxidative stress, and antioxidant therapy[J]. Oxidative medicine and cellular longevity, 2016, 2016(1): 1203285.
[5]. Klassen S S, Rabkin S W. The metalloporphyrin FeTPPS but not by cyclosporin A antagonizes the interaction of peroxynitrate and hydrogen peroxide on cardiomyocyte cell death[J]. Naunyn-Schmiedeberg's archives of pharmacology, 2009, 379(2): 149-161.
[6]. Uribe P, Barra J, Painen K, et al. FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa[J]. Antioxidants, 2023, 12(6): 1272.
[7]. Bruschetta G, Impellizzeri D, Campolo M, et al. FeTPPS reduces secondary damage and improves neurobehavioral functions after traumatic brain injury[J]. Frontiers in Neuroscience, 2017, 11: 6.
[8]. Duplain H, Sartori C, Dessen P, et al. Stimulation of peroxynitrite catalysis improves insulin sensitivity in high fat diet‐fed mice[J]. The Journal of physiology, 2008, 586(16): 4011-4016.
| Cell experiment [1]: | |
|
Cell lines |
Cardiomyocytes |
|
Preparation Method |
The MTT assay was used to assess cell viability. Cardiomyocytes in multiwell microtiter plates were treated for 24h with SIN-1, H2O2, FeTPPS, and/or cyclosporin A, as indicated. H2O2, 10 and 100μM, and cyclosporin A, 0.1 or 1.0μM. FeTPPS, 50μM, was chosen from concentration response data over a range. MTT dye was added to each well for the final 4h of the experiment, and solubilization solution was added to stop the reactions. As the absorbance curve of the MTT dye exhibits a linear portion ranging from 570 to 655nm, the absorbance was determined at 570nm on a multiwell plate reader using BioLinx 2.2 data capture software. Background absorbance of medium in the absence of cells was subtracted. All samples were assayed in duplicate, and the mean for each experiment was calculated. Experiments were conducted a minimum of five and a maximum of 14 times. |
|
Reaction Conditions |
50μM; 24h |
|
Applications |
FeTPPS reduces cell death caused by SIN-1 and SIN-1 plus H2O2. |
| Animal experiment [2]: | |
|
Animal models |
Controlled cortical impact injury (CCI) mice model |
|
Preparation Method |
In this study, a traumatic brain injury (TBI) model was established in mice using the CCI method. The mice were randomly divided into four groups (n=10 in each group): TBI+Vehicle group, TBI+FeTPPS group, Sham+Vehicle group, and Sham+FeTPPS group. TBI was performed by craniotomy on the right side and using an impactor to cause moderate brain injury (impact head diameter 4mm, depth 3mm, speed 1.5m/s); the Sham group only underwent craniotomy without impact. 1h and 4h after TBI or Sham surgery, normal saline or FeTPPS (30mg/kg, intraperitoneal injection) were administered, respectively. Postoperative behavioral tests such as Morris water maze, tail suspension, rotarod, and open field were performed, and brain water content, sway test, and blood sampling for biochemical indicators were measured 24h later. |
|
Dosage form |
10mg/kg, 30mg/kg; ip; two injections |
|
Applications |
FeTPPS reduces secondary damage and improves neurobehavioral functions after traumatic brain injury. |
|
References: |
|
| Cas No. | 90384-82-0 | SDF | |
| Chemical Name | Fe(III)5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato chloride | ||
| Canonical SMILES | [Cl-][Fe+3]123[N-]4C5=CC=C4C(C6=CC=C(S([O-])(=O)=O)C=C6)=C(C=C7)[N]1=C7C(C8=CC=C(S([O-])(=O)=O)C=C8)=C(C=C9)[N-]2C9=C(C%10=CC=C(S([O-])(=O)=O)C=C%10)C%11=[N]3C(C=C%11)=C5C%12=CC=C(S([O-])(=O)=O)C=C%12.[H+].[H+].[H+].[H+] | ||
| Formula | C44H24ClFeN4O12S4•4H | M.Wt | 1024.3 |
| Solubility | 5 mg/ml in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 976.3 μL | 4.8814 mL | 9.7628 mL |
| 5 mM | 195.3 μL | 976.3 μL | 1.9526 mL |
| 10 mM | 97.6 μL | 488.1 μL | 976.3 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)