GLPBio

FeTPPS

Catalog No.: GC43663Purity:>98.00%
COASDSData Sheet
FeTPPS is a metalloporphyrin-based ONOO⁻ decomposition catalyst.

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FeTPPS
Cas No.: 90384-82-0
50mgIn Stock
$76.00
1
100mgIn Stock
$142.00
1
250mgIn Stock
$276.00
1
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Description

FeTPPS is a metalloporphyrin-based ONOO⁻ decomposition catalyst [1]. FeTPPS catalyzes the decomposition of peroxynitrite (ONOO⁻), reducing its nitrating/oxidative damage to proteins, DNA, and lipids [2-3]. FeTPPS is primarily used in the study of oxidative stress-related disease models [4].

In cardiomyocytes, FeTPPS (50μM; 24h) reduces cell death caused by SIN-1 and SIN-1 plus H2O2 [5]. In spermatids, exposure to FeTPPS (25-50μM; 24h) ameliorates nitrosative stress [6].

In controlled cortical impact injury (CCI) mice model, FeTPPS (10mg/kg, 30mg/kg; ip; two injections) reduces secondary damage and improves neurobehavioral functions after traumatic brain injury. [7]. In a high-fat diet mice model, FeTPPS (50-60mg/kg; po; 8 weeks) treatment more than doubled the insulin-induced drop in blood glucose levels [8].

References:
[1]. Haber A, Gross Z. Catalytic antioxidant therapy by metallodrugs: lessons from metallocorroles[J]. Chemical Communications, 2015, 51(27): 5812-5827.
[2]. Uribe P, Barra J, Painen K, et al. FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa[J]. Antioxidants, 2023, 12(6): 1272.
[3]. Zhang P, Ma L, Yang Z, et al. 5, 10, 15, 20-Tetrakis (4-sulfonatophenyl) porphyrinato iron (III) chloride (FeTPPS), a peroxynitrite decomposition catalyst, catalyzes protein tyrosine nitration in the presence of hydrogen peroxide and nitrite[J]. Journal of Inorganic Biochemistry, 2018, 183: 9-17.
[4]. Duan X, Wen Z, Shen H, et al. Intracerebral hemorrhage, oxidative stress, and antioxidant therapy[J]. Oxidative medicine and cellular longevity, 2016, 2016(1): 1203285.
[5]. Klassen S S, Rabkin S W. The metalloporphyrin FeTPPS but not by cyclosporin A antagonizes the interaction of peroxynitrate and hydrogen peroxide on cardiomyocyte cell death[J]. Naunyn-Schmiedeberg's archives of pharmacology, 2009, 379(2): 149-161.
[6]. Uribe P, Barra J, Painen K, et al. FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa[J]. Antioxidants, 2023, 12(6): 1272.
[7]. Bruschetta G, Impellizzeri D, Campolo M, et al. FeTPPS reduces secondary damage and improves neurobehavioral functions after traumatic brain injury[J]. Frontiers in Neuroscience, 2017, 11: 6.
[8]. Duplain H, Sartori C, Dessen P, et al. Stimulation of peroxynitrite catalysis improves insulin sensitivity in high fat diet‐fed mice[J]. The Journal of physiology, 2008, 586(16): 4011-4016.

Protocol

Cell experiment [1]:

Cell lines

 Cardiomyocytes

Preparation Method

 The MTT assay was used to assess cell viability. Cardiomyocytes in multiwell microtiter plates were treated for 24h with SIN-1, H2O2, FeTPPS, and/or cyclosporin A, as indicated. H2O2, 10 and 100μM, and cyclosporin A, 0.1 or 1.0μM. FeTPPS, 50μM, was chosen from concentration response data over a range. MTT dye was added to each well for the final 4h of the experiment, and solubilization solution was added to stop the reactions. As the absorbance curve of the MTT dye exhibits a linear portion ranging from 570 to 655nm, the absorbance was determined at 570nm on a multiwell plate reader using BioLinx 2.2 data capture software. Background absorbance of medium in the absence of cells was subtracted. All samples were assayed in duplicate, and the mean for each experiment was calculated. Experiments were conducted a minimum of five and a maximum of 14 times.

Reaction Conditions

 50μM; 24h

Applications

 FeTPPS reduces cell death caused by SIN-1 and SIN-1 plus H2O2.
Animal experiment [2]:

Animal models

Controlled cortical impact injury (CCI) mice model

Preparation Method

In this study, a traumatic brain injury (TBI) model was established in mice using the CCI method. The mice were randomly divided into four groups (n=10 in each group): TBI+Vehicle group, TBI+FeTPPS group, Sham+Vehicle group, and Sham+FeTPPS group. TBI was performed by craniotomy on the right side and using an impactor to cause moderate brain injury (impact head diameter 4mm, depth 3mm, speed 1.5m/s); the Sham group only underwent craniotomy without impact. 1h and 4h after TBI or Sham surgery, normal saline or FeTPPS (30mg/kg, intraperitoneal injection) were administered, respectively. Postoperative behavioral tests such as Morris water maze, tail suspension, rotarod, and open field were performed, and brain water content, sway test, and blood sampling for biochemical indicators were measured 24h later.

Dosage form

 10mg/kg, 30mg/kg; ip; two injections

Applications

 FeTPPS reduces secondary damage and improves neurobehavioral functions after traumatic brain injury.

References:
[1]. Klassen S S, Rabkin S W. The metalloporphyrin FeTPPS but not by cyclosporin A antagonizes the interaction of peroxynitrate and hydrogen peroxide on cardiomyocyte cell death[J]. Naunyn-Schmiedeberg's archives of pharmacology, 2009, 379(2): 149-161.
[2]. Bruschetta G, Impellizzeri D, Campolo M, et al. FeTPPS reduces secondary damage and improves neurobehavioral functions after traumatic brain injury[J]. Frontiers in Neuroscience, 2017, 11: 6.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
90384-82-0
Chemical Name
Fe(III)5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato chloride
SMILES
[Cl-][Fe+3]123[N-]4C5=CC=C4C(C6=CC=C(S([O-])(=O)=O)C=C6)=C(C=C7)[N]1=C7C(C8=CC=C(S([O-])(=O)=O)C=C8)=C(C=C9)[N-]2C9=C(C%10=CC=C(S([O-])(=O)=O)C=C%10)C%11=[N]3C(C=C%11)=C5C%12=CC=C(S([O-])(=O)=O)C=C%12.[H+].[H+].[H+].[H+]
Molecular Formula
C44H24ClFeN4O12S4•4H
Molecular Weight
1024.3
Solubility
5 mg/ml in water
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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