Forchlorfenuron (Synonyms: CPPU, KT 30, N-Phenyl-N’-(2-chloro-4-pyridyl)urea) |
Catalog No.GC30279
One-Click Copy Product Info
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A plant growth regulator
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68157-60-8
Sample solution is provided at 25 µL, 10mM.
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Forchlorfenuron is plant growth regulator and cytokinin; can be used to increase fruit size of fruits, such as kiwi fruit and grapes.
Forchlorfenuron is a plant growth regulator, applied extensively to increase kiwifruit size and weight. Cytotoxicity of forchlorfenuron and its metabolites are tested through Sulforhodamine B assays against CHO cells. Forchlorfenuron exhibits significant cytotoxicity against CHO cells with an IC50 of 12.12±2.14 μM[1]. The half-lives offorchlorfenuron were 15.8-23.0 days, the final residues of forchlorfenuron in pulp were all ≤0.002 mg/kg, and most of the residues were concentrated in the peel. The risk assessment revealed that no significant potential health risk would be induced by forchlorfenuron in citrus fruits. Therefore, it could be safe to apply forchlorfenuron in citrus fruits[2].
[1]. Zhang Z, et al. Identification, synthesis, and safety assessment of forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) and its metabolites in kiwifruits. J Agric Food Chem. 2015 Mar 25;63(11):3059-66. [2]. Chen W, et al. Dissipation and residue of forchlorfenuron in citrus fruits. Bull Environ Contam Toxicol. 2013 Jun;90(6):756-60.
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Cell experiment: |
CHO cells are cultured in high-glucose DMEM medium. Forchlorfenuron and its kiwifruit metabolites are dissolved in DMSO and diluted in culture media. Cells are seeded in a 96-well flat microtiter plate for 24 h and are then incubated for an additional 48 h with various concentrations of the compounds. Next, cells are fixed with 10% trichloroacetic acid and incubated for 60 min at 4 °C. The supernatant is discarded, then the plates are washed. Using 0.4% SRB solution dissolved in 1% acetic acid, the cell layer is strained, then the plates are incubated for 20 min at room temperature. The stained cells is solubilized in 10 mM un-buffered Tris base and optical density (OD) is measured at 560 nm[1]. |
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References: [1]. Zhang Z, et al. Identification, synthesis, and safety assessment of forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) and its metabolites in kiwifruits. J Agric Food Chem. 2015 Mar 25;63(11):3059-66. |
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| Cas No. | 68157-60-8 | SDF | |
| Synonyms | CPPU, KT 30, N-Phenyl-N’-(2-chloro-4-pyridyl)urea | ||
| Canonical SMILES | O=C(NC1=CC=CC=C1)NC2=CC(Cl)=NC=C2 | ||
| Formula | C12H10ClN3O | M.Wt | 247.68 |
| Solubility | DMSO : ≥ 29 mg/mL (117.09 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0375 mL | 20.1873 mL | 40.3747 mL |
| 5 mM | 807.5 μL | 4.0375 mL | 8.0749 mL |
| 10 mM | 403.7 μL | 2.0187 mL | 4.0375 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)