Alexa Fluor 594 labeled Concanavalin A (Synonyms: Concanavalin Afrom Canavalla ensiformis (Jack bean), Alexa Fluor 594 ConiugateAlexa Fluor 594-ConA:Alexa Fluor 594 labeled Concan avalin A (ConA): ConAlectin(Alexa Fluor 594):) |
| Catalog No.GC26242 |
Concanavalin ACon A, derived from Canavalia ensiformis beans, is a lectin protein (Mw 104 kDa).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11028-71-0
Sample solution is provided at 25 µL, 10mM.
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Concanavalin ACon A, derived from Canavalia ensiformis beans, is a lectin protein (Mw 104 kDa). Under the condition of p27.0, Con A exists in a homogenous tetrameric structure, consisting of four subunits with a molecular weight of 26 kDa. Under acidic conditions (pH 4.5-5.5), on A dissociates into an activated dimeric structure of 52 kDa. Each monomer, regardless of pH or molecular structure, contains two metal case binding sites. Metal nanoparticles C82+and M2+must bind to both sites in order for Cn A to be active. Awakening is the process of generating a stable form of dimeric structure from other living organisms.
Concanavalin A (Con A) has no blood type specificity and selectively binds to - mannose/residues and a-glucose residues on various proteins, sugars, and class molecules. It requires the presence of Ca2+and Mn2+ions to display its activity. Con A is suitable for carbohydrate research, glycoprotein purification, enzyme labeling, thin membrane studies, Shao cell agglutination, and cell typing studies.
This is Alexa Fluor 594 labeled Concanavalin A (Alexa Fluor 594 ConA), purified by affinity purification at Ex/Em=590/617nm. The recommended working concentration range is 5-25 μ G/ml.
Operation process
1、 Preparation of storage solution
Before use, place Alexa Fluor 594-ConA (1mg) stored at low temperature for 30 minutes at room temperature, leave at low speed for 2-3 minutes, and add 500ul ddH2O (sterile) to prepare a 2mg/ml storage solution. Short term use can be stored at 2-8 ° C, and long-term use should be packaged and stored at -20 ° C according to a single dose to avoid repeated freeze-thaw cycles. It should be stable for at least one month.
2、 Live cell staining
[Note 1] Before use, place the low-temperature stored product at room temperature to completely melt it, then centrifuge at low speed and extract the supernatant for subsequent experiments. This step can remove protein aggregates that may form during the storage process of the product, thereby reducing non-specific background staining.
[Note 2] Cona protein conjugates (such as Alexa Fluor 594 ConA) stain the cytoplasmic membrane and ultimately stain the intracellular vesicles after invagination of the membrane
3、 Fixed cell staining
[Note 1] Before use, place the low-temperature stored product at room temperature to completely melt it, then centrifuge at low speed and absorb the supernatant for subsequent experiments. This step can remove protein aggregates that may form during the storage process of this product, thereby reducing non-specific background staining.
[Note 2] Cona protein conjugates (such as Alexa Fuor 594 ConA) do not exhibit membrane permeability, therefore, fixed (non permeable) cells exhibit cytoplasmic membrane staining. If fine fixed and then subjected to chemical treatment, it may cause staining of the cytoplasmic membrane.
matters needing attention
1) Long term storage of this product may result in precipitation. Please incubate at 37 ℃ for a few minutes before use, and then centrifuge and extract the supernatant for use. This operation will not have a negative impact on performance.
2) Fluorescent dyes all have quenching problems, so be careful to avoid light during storage and operation.
3) For your safety and health, please wear laboratory clothes and disposable gloves when operating.
| Cas No. | 11028-71-0 | SDF | |
| Synonymes | Concanavalin Afrom Canavalla ensiformis (Jack bean), Alexa Fluor 594 ConiugateAlexa Fluor 594-ConA:Alexa Fluor 594 labeled Concan avalin A (ConA): ConAlectin(Alexa Fluor 594): | ||
| Formula | M.Wt | 104kDa | |
| Solubility | H2O : 8.33 mg/mL (Need ultrasonic) | Storage | Store at -20°C,protect from light,valid for at least 1 year |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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