CM-H2DCFDA |
Catalog No.GC71777
One-Click Copy Product Info
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CM-H2DCFDA est un dérivé de h2dcfda.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 850013-49-9
Sample solution is provided at 25 µL, 10mM.
Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ROS Measurement:1. Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
References:
[1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11.
[2]. Kolarova H, et al. Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. Gen Physiol Biophys. 2008 Jun;27(2):101-5.
| Cas No. | 850013-49-9 | SDF | |
| Formula | C27H19Cl3O8 | M.Wt | 577.79 |
| Solubility | Storage | 4°C, protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7307 mL | 8.6537 mL | 17.3073 mL |
| 5 mM | 346.1 μL | 1.7307 mL | 3.4615 mL |
| 10 mM | 173.1 μL | 865.4 μL | 1.7307 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >92.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)