egg white lysozyme (19-36) [Gallus gallus] (Synonyms: H2N-Lys-Val-Phe-Gly-Arg-Cys-Glu-Leu-Ala-Ala-Ala-Met-Lys-Arg-His-Gly-Leu-Asp-OH ) |
| Catalog No.GP10136 |
Dissolution of cell wall
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![egg white lysozyme (19-36) [Gallus gallus] Chemical Structure](/media/struct/GP1/GP10136.png "egg white lysozyme (19-36) [Gallus gallus] Chemical Structure")
Sample solution is provided at 25 µL, 10mM.
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Sequence: H2N-KVFGRCELAAAMKRHGLD-OH
Formula of Egg white lysozyme (19-36) [Gallus gallus]:
C86H144N28O23S2
Hen egg white lysozyme has a molecular weight of about 14,600 and each molecule comprises 129 amino acid residues1.
The crystals have unit cell dimensions a = b = 79.1, c = 37.9 A and space group P43212, and there are eight molecules in the unit cell, one per asymmetric unit2. Liquid of crystallization constitutes about 33.5 per cent of the weight of the crystal3.
The activity of lysozyme in promoting the dissolution of bacterial cell walls is now known to depend upon its ability to catalyze the hydrolysis of ,3-(1-4) glycosidic linkages between amino sugar residues in poly-saccharide components of these cell walls1.
References:
1. David C. Phillips. The Hen Egg-White Lysozyme Molecule. (1967) Proceedings of the National Academy of Sciences of the United States of America, 57: 483-495
2. Blake, C. C. F., E. H. Fenn, A. C. T. North, D. C. Phillips, and t. J. Polijak, Nature, 196, 1173 (1962).
3. Steinrauf, L. K., Acta Cryst., 12, 77 (1959).
| Cas No. | SDF | ||
| Synonymes | H2N-Lys-Val-Phe-Gly-Arg-Cys-Glu-Leu-Ala-Ala-Ala-Met-Lys-Arg-His-Gly-Leu-Asp-OH | ||
| Formula | C86H144N28O23S2 | M.Wt | 2002.37 |
| Solubility | ≥402.3mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.4994 mL | 2.497 mL | 4.9941 mL |
| 5 mM | 0.0999 mL | 0.4994 mL | 0.9988 mL |
| 10 mM | 0.0499 mL | 0.2497 mL | 0.4994 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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