Laminin (925-933) (Synonyms: CDPGYIGSR, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg) |
Catalog No.GP10103 |
Laminine (925-933) (CDPGYIGSR), est la séquence de Laminine sur la chaîne B1.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 110590-60-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Laminin coating of culture plates: For coating experiments, all laminin preparations were reconstituted in sterile PBS and then diluted to 10 μg/ml in PBS. Each laminin preparation was adsorbed to 6-well plates for 6 hrs at room temperature. Non-specific binding sites were blocked for 30 minutes with PBS containing 0.1% w/v BSA, at room temperature. Prior to seeding of cells onto the laminin-coated plates, the plates were washed with DMEM. This protocol only provides a guideline, and should be modified according to your specific needs.
|
References: [1] Tran T, et al. Endogenous laminin is required for human airway smooth muscle cell maturation. Respir Res. 2006 Sep 12;7(1):117. |
Laminine (925-933) (CDPGYIGSR), est la séquence de Laminine sur la chaîne B1. YIGSR est actif dans la promotion de l'adhérence d'une variété de cellules épithéliales[1]. La laminine se lie au collagène IV, à la protéoglycane sulfate d'héparane et au nidogen-entactin, et elle médiatise les interactions cellulaires avec cette matrice[2].
Un peptide synthétique dérivé de la laminine, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2 (CDPGYIGSR-H2), contenant un site actif pour la liaison cellulaire a inhibé à la fois l'angiogenèse et la croissance des tumeurs solides[3].
In vitro, à des concentrations de 100 et 300 µg/ml, la laminine (925-933) peut stimuler l'attachement des cellules HT-1080 et CHO aux plaques de culture[2].
In vitro, le domaine oligopeptide Laminin (925-933) lié de manière covalente à un gel d'agarose en tant que substrat bioartificiel 3D supporte avec succès la croissance des neurites provenant des ganglions racines dorsales (DRG)[2]. In vitro, une réduction de 78% de l'attachement cellulaire a été observée sur les films contenant du Laminin (925-933) dans le milieu de culture cellulaire[4].
Des expériences in vivo ont révélé que le gel dérivé de la Laminine (925-933) avait la capacité d'améliorer la régénération nerveuse dans un modèle de racine dorsale de rat sectionnée par rapport à un gel non dérivé, un gel de Laminine (925-933) et des canaux de guidage nerveux remplis de solution saline[3].
References:
[1] Graf J, et al. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987 Nov 3;26(22):6896-900.
[2] Graf J, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell. 1987 Mar 27;48(6):989-96.
[3] Borkenhagen M, et al. Three-dimensional extracellular matrix engineering in the nervous system. J Biomed Mater Res. 1998 Jun 5;40(3):392-400.
[4] Ranieri JP, et al. Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates. Int J Dev Neurosci. 1994 Dec;12(8):725-35.
Cas No. | 110590-60-8 | SDF | |
Synonymes | CDPGYIGSR, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg | ||
Chemical Name | Laminin (925-933) | ||
Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CO)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)CNC(=O)C2CCCN2C(=O)C(CC(=O)O)NC(=O)C(CS)N | ||
Formula | C40H62N12O14S | M.Wt | 967.06 |
Solubility | ≥48.35mg/mL in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.0341 mL | 5.1703 mL | 10.3406 mL |
5 mM | 0.2068 mL | 1.0341 mL | 2.0681 mL |
10 mM | 0.1034 mL | 0.517 mL | 1.0341 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *