Handle region peptide, rat

Cell experiment:

The immortalized rat glomerular mesangial cells are incubated at 37°C in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% bovine calf serum (BCS) in a humidified atmosphere of 95% air plus 5% CO2. When the cell reaches about 80% confluence, they are made quiescent by decreasing BCS to 0.5% for 24 to 48 h. To detect the effect of Handle region peptide on p44/42 MAP kinase, the cells are further cultured in serum-deprived medium for 24 h before 1 µM Handle region peptide is added in. The cells are harvested at 0, 1, 5, 10, 60, 120 min time points after Handle region peptide application. Protein isolated from cell lyses is used for subsequent Western blotting analysis[1].

Animal experiment:

Rats[2]Long-Evans rats (5-7 weeks old) are treated with 0.1-mL intraperitoneal injections of vehicle (PBS) or Handle region peptide (0.1 or 0.01 mg/kg body weight) the day before and immediately after the injection of LPS. The effects of Handle region peptide treatment on ocular inflammation are evaluated 24 hours after LPS injection[2].

References:

[1]. He M, et al. Inhibition of renin/prorenin receptor attenuated mesangial cell proliferation and reduced associated fibrotic factor release. Eur J Pharmacol. 2009 Mar 15;606(1-3):155-61.
[2]. Satofuka S, et al. Suppression of ocular inflammation in endotoxin-induced uveitis by inhibiting nonproteolytic activation of prorenin. Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2686-92.