Im-m7GDP sodium is a capping reagent and also the P-imidazolide of N7-methylguanosine 5'-diphosphate. Im-m7GDP sodium introduces an m7G cap structure to the monophosphate group of branched oligoribonucleotides, and is used in nucleic acid synthesis-related research [1] [2] [3].
In Vitro, Im-m7GDP (10 mM; 3 h) sodium efficiently introduces an m7G cap structure to the 5′ phosphorylated branched strand of synthetic oligoribonucleotides under the specified reaction conditions[2]. Im-m7GDP (10 mM; 3 h) sodium effectively introduces a cap structure onto 5′-monophosphorylated 68-nt chemically synthesized RNA in a cell-free in vitro reaction[3].
References:
[1]. Geng J, et al. Cap-related modifications of RNA regulate binding to IFIT proteins. RNA (New York, N.Y.). 2024 Sep 16;30(10):1292-1305.
[2]. Fukuchi K, et al. Internal cap-initiated translation for efficient protein production from circular mRNA. Nature biotechnology. 2026 Jan;44(1):120-132.
[3]. Ohashi S, et al. Selection of Short 5'-UTR of Chemically Synthesized mRNA to Improve Translation Efficiency. Chemical & pharmaceutical bulletin. 2025;73(5):449-456.
In Vitro, Im-m7GDP (10 mM; 3 h) sodium efficiently introduces an m7G cap structure to the 5′ phosphorylated branched strand of synthetic oligoribonucleotides under the specified reaction conditions[2]. Im-m7GDP (10 mM; 3 h) sodium effectively introduces a cap structure onto 5′-monophosphorylated 68-nt chemically synthesized RNA in a cell-free in vitro reaction[3].
References:
[1]. Geng J, et al. Cap-related modifications of RNA regulate binding to IFIT proteins. RNA (New York, N.Y.). 2024 Sep 16;30(10):1292-1305.
[2]. Fukuchi K, et al. Internal cap-initiated translation for efficient protein production from circular mRNA. Nature biotechnology. 2026 Jan;44(1):120-132.
[3]. Ohashi S, et al. Selection of Short 5'-UTR of Chemically Synthesized mRNA to Improve Translation Efficiency. Chemical & pharmaceutical bulletin. 2025;73(5):449-456.