Stains-All |
| Catalog No.GC30230 |
Stains-All, un colorant carbocyanine cationique, est une sonde pratique pour étudier les caractéristiques structurelles des sites individuels de liaison au calcium de la calmoduline (CaM) et des protéines de liaison au calcium apparentées (CaBP) .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 7423-31-6
Sample solution is provided at 25 µL, 10mM.
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Stains-All is a cationic carbocyanine dye.
Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue[1].
[1]. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983 Sep 25;258(18):11267-73.
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