trans-Zeatin |
Catalog No.GC30162
One-Click Copy Product Info
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trans-Zeatin est une cytokinine naturelle, une phytohormone qui joue un rôle central dans la croissance, la différenciation et la division des cellules végétales.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1637-39-4
Sample solution is provided at 25 µL, 10mM.
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trans-Zeatin est une cytokinine naturelle, une phytohormone qui joue un rôle central dans la croissance, la différenciation et la division des cellules végétales[1, 2]. trans-Zeatin peut être utilisée comme adfitif dans les milieux de culture pour induire la croissance de cals et la formation de bourgeons[3]. trans-Zeatin est une substance active potentielle pour le traitement du photo-vieillissement cutané[4].
In vitro, le traitement de fibroblastes de peau humaine (FPH) avec trans-Zeatin (20-80μM) pendant 24 heures a augmenté la viabilité cellulaire des FPH irradiés aux UVB et significativement inhibé l'expression de la métallogprotéinsase matricielle-1 (MMP-1) induite par UVB[5]. Le traitement de kératinocytes cutanés (cellules HaCaT) avec trans-Zeatin (20-80μM) pendant 4 à 24 heures a régulé à la hasse l'expression de l' aquaporine-3 (AQP3) dans les cellules HaCaT, de manière dépendante du temps et de la dose[6].
References:
[1] Schäfer M, Brütting C, Meza-Canales I D, et al. The role of cis-zeatin-type cytokinins in plant growth regulation and mediating responses to environmental interactions[J]. Journal of experimental botany, 2015, 66(16): 4873-4884.
[2] Großkinsky D, Edelsbrunner K, Pfeifhofer H, et al. Cis-and trans-zeatin differentially modulate plant immunity[J]. Plant signaling & behavior, 2013, 8(7): e24798.
[3] Cardoza V. Tissue culture: The manipulation of plant development[J]. Plant Biotechnology and Genetics: principles, techniques, and applications, 2008: 113-134.
[4] Kesharwani A, Panda S P. Isolation of Phytohormone trans-Zeatin: Potential Oxidant Scavenger and Anti-Aging Compound[J]. Neurochemical Journal, 2024, 18(1): 134-146.
[5] Yang B, Ji C, Kang J, et al. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts[J]. International Journal of Molecular Medicine, 2009, 23(4): 555-560.
[6] Ji C, Yang Y, Yang B, et al. Trans-Zeatin attenuates ultraviolet induced down-regulation of aquaporin-3 in cultured human skin keratinocytes[J]. International journal of molecular medicine, 2010, 26(2): 257-263.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Fibroblastes de peau humaine (FPH) |
Méthode de préparation | Des fibroblastes de peau humaine (FPH) ont été encemencés dans une plaque de 96 puits. Après des traitements avec diverses concentrations de trans-Zeatin (20-80μM) ou avec UVB (30mJ/cm2), les cellules ont été cultivées dans 100μL de DMEM supplémenté avec 10% de sérum dans chaque puits pendant 24h. La viabilité cellulaire a été déterminée à l'aide du test MTT. |
Conditions de réaction | 20-80μM; 24h |
Domaines d'application | trans-Zeatin (20-80μM) a augmenté la viabilité cellulaire des fibroblastes de peau humaine (FPH) irradiés par UVB. |
References: | |
| Cas No. | 1637-39-4 | SDF | |
| Canonical SMILES | OC/C(C)=C/CNC1=C2N=CNC2=NC=N1 | ||
| Formula | C10H13N5O | M.Wt | 219.24 |
| Solubility | DMSO : ≥ 60 mg/mL (273.67 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5612 mL | 22.8061 mL | 45.6121 mL |
| 5 mM | 912.2 μL | 4.5612 mL | 9.1224 mL |
| 10 mM | 456.1 μL | 2.2806 mL | 4.5612 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)