WYneC (Synonyms: Wittig-alkyne C) |
Catalog No.GC91968
One-Click Copy Product Info
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WYneC est une sonde chimiquement sélective pour la détection des protéines sulfonylées d'Asie centrale dans les cellules vivantes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 74763-44-3
Sample solution is provided at 25 µL, 10mM.
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WYneC is a chemoselective probe for the detection of sulfenylated proteins in live cells.1,2 It is selective for a sulfenylated dipeptide over sulfinic acid- or sulfonic acid-containing dipeptides, reduced glutathione (GSH), glutathione disulfide (GSSG), and S-nitrosoglutathione (GSNO), as well as cysteine sulfinic acid and glutathione sulfonic acid, in cell-free assays.1 WYneC also selectively binds to C64,82S glutathione peroxidase 3 (GPX3) containing a sulfenic acid at Cys36 over C64,82S GPX3 containing a reduced thiol, sulfinic acid at Cys36, or an intramolecular disulfide bond in cell-free assays. It binds to sulfenylated proteins in live cells and can then be conjugated to azide-containing fluorescent probes for detection.
References:
[1]. Shi, Y., Fu, L., Yang, J., et al.Wittig reagents for chemoselective sulfenic acid ligation enables global site stoichiometry analysis and redox-controlled mitochondrial targetingNat. Chem.13(11)1140-1150(2021).
[2]. He, J.-X., Fei, Z.-C., Fu, L., et al.A modification-centric assessment tool for the performance of chemoproteomic probesNat. Chem. Biol.18(8)904-912(2022).
| Cas No. | 74763-44-3 | SDF | |
| Synonymes | Wittig-alkyne C | ||
| Chemical Name | 1-(triphenylphosphoranylidene)-5-hexyn-2-one | ||
| Canonical SMILES | O=C(CCC#C)C=P(C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3 | ||
| Formula | C24H21OP | M.Wt | 356.4 |
| Solubility | DMSO: Sparingly soluble: 1-10 mg/ml,Ethanol: Sparingly soluble: 1-10 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8058 mL | 14.0292 mL | 28.0584 mL |
| 5 mM | 561.2 μL | 2.8058 mL | 5.6117 mL |
| 10 mM | 280.6 μL | 1.4029 mL | 2.8058 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)