GLPBio

H-Gly-Pro-Hyp-OH

Catalog No.: GA22509Purity:>98.00%
COASDSData Sheet
H-Gly-Pro-Hyp-OH is an oral collagen tripeptide with high enzyme stability and intestinal permeability.

Products are for research use only. Not for human use. We do not sell to patients.

H-Gly-Pro-Hyp-OH
Cas No.: 2239-67-0
25mgIn Stock
$38.00
1
50mgIn Stock
$60.00
1
100mgIn Stock
$95.00
1
250mgIn Stock
$151.00
1
500mgIn Stock
$235.00
1
1gIn Stock
$353.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

No citations are currently available for this product. Below are selected publications citing GlpBio products.
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

Description

H-Gly-Pro-Hyp-OH is an oral collagen tripeptide with high enzyme stability and intestinal permeability [1]. H-Gly-Pro-Hyp-OH is a moderately competitive inhibitor of dipeptidylpeptidase-IV with a Ki value of 4.5mM[2]. H-Gly-Pro-Hyp-OH has unique specificity for platelet Gp VI and mediates the activation of platelets by collagen [3]. H-Gly-Pro-Hyp-OH treatment inhibits the excessive growth of S. mutans and promotes the dominant growth of S. oralis Dominance [4]. H-Gly-Pro-Hyp-OH has been widely used to improve skin conditions and regulate the central nervous system [5].

In vivo, H-Gly-Pro-Hyp-OH treatment via oral administration at a dose of 31.36mg/kg/day for 6 weeks increased the content of elastin in the skin of aged mice exposed to light, and alleviated the damage to the skin structure on the back caused by chronic ultraviolet radiation[6].

References:
[1] Sontakke S B, Jung J, Piao Z, et al. Orally available collagen tripeptide: enzymatic stability, intestinal permeability, and absorption of Gly-Pro-Hyp and Pro-Hyp[J]. Journal of agricultural and food chemistry, 2016, 64(38): 7127-7133.
[2] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[3] Knight C G, Morton L F, Onley D J, et al. Collagen–platelet interaction: Gly-Pro-Hyp is uniquely specific for platelet Gp VI and mediates platelet activation by collagen[J]. Cardiovascular research, 1999, 41(2): 450-457.
[4] Jung H Y, Cai J N, Yoo S C, et al. Collagen peptide in a combinatorial treatment with Lactobacillus rhamnosus inhibits the cariogenic properties of Streptococcus mutans: An in vitro study[J]. International Journal of Molecular Sciences, 2022, 23(3): 1860.
[5] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[6] Yang D, Liu Q, Xu Q, et al. Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide[J]. Food & function, 2024, 15(6): 3008-3022.

Protocol

Animal experiment [1]:

Animal models

 Female Kunming mice

Preparation Method

 Female Kunming mice (4 weeks old), weighing 18-22g were kept in temperature-controlled (21°C-25°C) and humidity-maintained (50%-60%) rooms with a 12-h light/dark cycle. Water and food were provided freely. All mice were acclimatized for a week before the experiments. After acclimation, the mice were randomly divided into 3 groups (n=10) as follows: (i) normal control group, UV-unexposed, (ii) UV group, (iii) H-Gly-Pro-Hyp-OH group (31.36mg/kg/day; p.o.). Except for the normal control group, the dorsal skins of mice in the other groups were irradiated with four UVA and two UVB lamps three times a week for a total of six weeks. Before each irradiation, the dorsal skin hair was removed using a shaver, followed by applying hair removal cream to the skin for thorough depilation. Appropriate cages were used to prevent mice from moving during irradiation. The irradiation intensity was measured with a radiometer to determine the minimal erythema dose (MED) and the distance between the skins and lamps was 30cm. The irradiation dose was 0.5MED/time in the first week, 1.0MED/time in the second week, and was escalated by 0.5MED/time per week until 3.0MED/time in the sixth week. All the oral administrations began 30min before irradiation. At the end of the photoaging test, the mice were sacrificed. Appropriate dorsal skin tissues were taken for histological staining.

Dosage form

 31.36mg/kg/day for 6 weeks; p.o.

Applications

 H-Gly-Pro-Hyp-OH treatment increased the content of elastin in the skin of aged mice exposed to UV.

References:
[1] Yang D, Liu Q, Xu Q, et al. Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide[J]. Food & function, 2024, 15(6): 3008-3022.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
2239-67-0
Molecular Formula
C12H19N3O5
Molecular Weight
285.3
Solubility
Soluble in DMSO
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

Reviews

Review for H-Gly-Pro-Hyp-OH

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%