H-Gly-Pro-Hyp-OH |
Catalog No.GA22509
One-Click Copy Product Info
|
H-Gly-Pro-Hyp-OH is an oral collagen tripeptide with high enzyme stability and intestinal permeability.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2239-67-0
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
H-Gly-Pro-Hyp-OH is an oral collagen tripeptide with high enzyme stability and intestinal permeability [1]. H-Gly-Pro-Hyp-OH is a moderately competitive inhibitor of dipeptidylpeptidase-IV with a Ki value of 4.5mM[2]. H-Gly-Pro-Hyp-OH has unique specificity for platelet Gp VI and mediates the activation of platelets by collagen [3]. H-Gly-Pro-Hyp-OH treatment inhibits the excessive growth of S. mutans and promotes the dominant growth of S. oralis Dominance [4]. H-Gly-Pro-Hyp-OH has been widely used to improve skin conditions and regulate the central nervous system [5].
In vivo, H-Gly-Pro-Hyp-OH treatment via oral administration at a dose of 31.36mg/kg/day for 6 weeks increased the content of elastin in the skin of aged mice exposed to light, and alleviated the damage to the skin structure on the back caused by chronic ultraviolet radiation[6].
References:
[1] Sontakke S B, Jung J, Piao Z, et al. Orally available collagen tripeptide: enzymatic stability, intestinal permeability, and absorption of Gly-Pro-Hyp and Pro-Hyp[J]. Journal of agricultural and food chemistry, 2016, 64(38): 7127-7133.
[2] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[3] Knight C G, Morton L F, Onley D J, et al. Collagen–platelet interaction: Gly-Pro-Hyp is uniquely specific for platelet Gp VI and mediates platelet activation by collagen[J]. Cardiovascular research, 1999, 41(2): 450-457.
[4] Jung H Y, Cai J N, Yoo S C, et al. Collagen peptide in a combinatorial treatment with Lactobacillus rhamnosus inhibits the cariogenic properties of Streptococcus mutans: An in vitro study[J]. International Journal of Molecular Sciences, 2022, 23(3): 1860.
[5] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[6] Yang D, Liu Q, Xu Q, et al. Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide[J]. Food & function, 2024, 15(6): 3008-3022.
| Animal experiment [1]: | |
|
Animal models |
Female Kunming mice |
|
Preparation Method |
Female Kunming mice (4 weeks old), weighing 18-22g were kept in temperature-controlled (21°C-25°C) and humidity-maintained (50%-60%) rooms with a 12-h light/dark cycle. Water and food were provided freely. All mice were acclimatized for a week before the experiments. After acclimation, the mice were randomly divided into 3 groups (n=10) as follows: (i) normal control group, UV-unexposed, (ii) UV group, (iii) H-Gly-Pro-Hyp-OH group (31.36mg/kg/day; p.o.). Except for the normal control group, the dorsal skins of mice in the other groups were irradiated with four UVA and two UVB lamps three times a week for a total of six weeks. Before each irradiation, the dorsal skin hair was removed using a shaver, followed by applying hair removal cream to the skin for thorough depilation. Appropriate cages were used to prevent mice from moving during irradiation. The irradiation intensity was measured with a radiometer to determine the minimal erythema dose (MED) and the distance between the skins and lamps was 30cm. The irradiation dose was 0.5MED/time in the first week, 1.0MED/time in the second week, and was escalated by 0.5MED/time per week until 3.0MED/time in the sixth week. All the oral administrations began 30min before irradiation. At the end of the photoaging test, the mice were sacrificed. Appropriate dorsal skin tissues were taken for histological staining. |
|
Dosage form |
31.36mg/kg/day for 6 weeks; p.o. |
|
Applications |
H-Gly-Pro-Hyp-OH treatment increased the content of elastin in the skin of aged mice exposed to UV. |
|
References: |
|
| Cas No. | 2239-67-0 | SDF | |
| Formula | C12H19N3O5 | M.Wt | 285.3 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5051 mL | 17.5254 mL | 35.0508 mL |
| 5 mM | 701 μL | 3.5051 mL | 7.0102 mL |
| 10 mM | 350.5 μL | 1.7525 mL | 3.5051 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)