Silymarin |
Catalog No.GC19946
One-Click Copy Product Info
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Silymarin is an effective SARS-CoV-2 main protease (Mpro) inhibitor. Silymarin displays antioxidant and membrane stabilizing activity. It protects various tissues and organs against chemical injury. Silymarin can reduce tumor cell proliferation, angiogenesis as well as insulin resistance.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 65666-07-1
Sample solution is provided at 25 µL, 10mM.
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Silymarin is an effective SARS-CoV-2 main protease (Mpro) inhibitor. Silymarin displays antioxidant and membrane stabilizing activity. It protects various tissues and organs against chemical injury. Silymarin can reduce tumor cell proliferation, angiogenesis as well as insulin resistance[1].
Silymarin (0, 40, 80µg/ml; 24h) was confirmed to inhibit migration of AGS cells in a concentration-dependent manner (59.4% at 40µg/ml and 21.7% at 80µg/ml) [2]. Silymarin(0, 25, 50, 75, 100, 150 and 200µg/ml; 24h) inhibits the viability of MDA-MB-231 and MCF-7 human breast cancer cells, and MCF-7 cells are more sensitive to Silymarin than MDA-MB-231 cells[3].
Silymarin (25, 50mg/kg; po; 5 times per week; 21d) inhibits the growth of MCF-7 tumors and induces apoptosis of MCF-7 tumor cells[3]. Silymarin (10, 20, 50 and 100mg/kg; po; administered 60 minutes before behavioral testing) reduces the immobility time in the forced swim test and the tail suspension test in a dose-dependent manner[4].
References:
[1].Féher J, Lengyel G. Silymarin in the prevention and treatment of liver diseases and primary liver cancer. Curr Pharm Biotechnol. 2012 Jan;13(1):210-7.
[2].Kim SH, Choo GS, Yoo ES, Woo JS, Han SH, Lee JH, Jung JY. Silymarin induces inhibition of growth and apoptosis through modulation of the MAPK signaling pathway in AGS human gastric cancer cells. Oncol Rep. 2019 Nov;42(5):1904-1914.
[3]. Kim S H, Choo G S, Yoo E S, et al. Silymarin inhibits proliferation of human breast cancer cells via regulation of the MAPK signaling pathway and induction of apoptosis[J]. Oncology Letters, 2021, 21(6): 1-10.
[4]. Khoshnoodi M, Fakhraei N, Dehpour AR. Possible involvement of nitric oxide in antidepressant-like effect of silymarin in male mice. Pharm Biol. 2015 May;53(5):739-45.
| Cell experiment [1]: | |
Cell lines | MDA-MB-231 and MCF-7 cells |
Preparation Method | MDA-MB-231 and MCF-7 cells were treated with Silymarin0, 25, 50, 75, 100, 150 and 200µg/ml for 24h. Silymarin-induced changes in breast cancer cell viability were assessed using a MTT assay. |
Reaction Conditions | 0, 25, 50, 75, 100, 150 and 200µg/ml; 24h |
Applications | Silymarin inhibits the viability of MDA-MB-231 and MCF-7 human breast cancer cells, and MCF-7 cells are more sensitive to Silymarinthan MDA-MB-231 cells. |
| Animal experiment [1]: | |
Animal models | Xenograft Tumor Model |
Preparation Method | Silymarin was dissolved in alcohol and diluted with distilled water and then orally administered 5 times per week at a dose of 25 or 50mg/kg body weight, while the control group mice were administered the vehicle (distilled water) and the same amount of alcohol that used to dissolve the Silymarin. Tumor weight and size were measured twice a week for a total of 21 days. |
Dosage form | 25, 50mg/kg; 5 times per week; 21d |
Applications | Silymarin has an inhibitory effect on MCF-7 tumor growth and induced apoptosis in the MCF-7 tumors. |
References: | |
| Cas No. | 65666-07-1 | SDF | |
| Formula | C25H22O10 | M.Wt | 482.44 |
| Solubility | DMSO: 120 mg/mL (Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0728 mL | 10.364 mL | 20.728 mL |
| 5 mM | 414.6 μL | 2.0728 mL | 4.1456 mL |
| 10 mM | 207.3 μL | 1.0364 mL | 2.0728 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >80.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)